Application of Plastic Strain Method to Determine Threshold Concentrations for Cryoprotective Substances During Saccharomyces cerevisiae Yeast Cell Cryopreservation

Authors

  • A. I. Osetsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. L. Kirilyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. P. Vysekantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

plastic strain, fluidity threshold, stress-strain curves, glycerol, DMSO, PEO-1500, Saccharomyces cerevisiae

Abstract

Plastic strain of glycerol and PEO-1500 frozen aqueous solutions has been studied and an alteration in their plastic characteristics when achieving certain (threshold) concentrations of cryoprotective substances has been established. This change is associated to local closed liquid phase inclusion transition into the through liquid interlayers, that excludes an internal pressure development and corresponding mechanical damages in biological objects being under cryopreservation. This conclusion is confirmed by the experiments on Saccharomyces cerevisiae yeast cell cryopreservation.

Published

2007-03-15

How to Cite

Osetsky, A. I., Kirilyuk, A. L., Gurina, T. M., & Vysekantsev, I. P. (2007). Application of Plastic Strain Method to Determine Threshold Concentrations for Cryoprotective Substances During Saccharomyces cerevisiae Yeast Cell Cryopreservation. Problems of Cryobiology and Cryomedicine, 17(1), 71-79. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/534

Issue

Section

Theoretical and Experimental Cryobiology