Cryopreservation of Human Fetal Fibroblast Culture

Authors

  • V. V. Timon Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. F. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. I. Goncharuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

human fetal fibroblast culture, 1, 2-propanediol, oxyethylated glycerol, cryopreservation

Abstract

Cryoprotective properties of 1,2-propanediol (1,2-PD), oxyethylated glycerol (OEG; n=5) and fetal calf serum (FCS) when freeze-thaw human fetal fibroblast (HFF) culture were studied. It has been established that 1,2-PD and OEG have weak cryoprotective properties and do not provide the integrity of functional activity of HEF cells. Cell cryopreservation in FCS provided maximum integrity of cells and their functional activity.

Published

2007-03-15

How to Cite

Timon, V. V., Petrenko, T. F., & Goncharuk, E. I. (2007). Cryopreservation of Human Fetal Fibroblast Culture. Problems of Cryobiology and Cryomedicine, 17(1), 80-85. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/535

Issue

Section

Cryopreservation of Biological Resources