Effect of Storage of Placenta Tissue at –20°C on Properties of Its Aqueous and Saline Extracts

Oleg A. Nardid, Ekaterina D. Rozanova, Yana O. Cherkashina, Svetlana V. Repina, Eduard O. Nardid


Research was aimed to study the effect of human placenta tissue storage at –20°C on the features of procured from it aqueous-saline extracts and their certain fractions. The methods of gel-chromatography, optical spectroscopy and spin probe EPR were used in the research. Spin probe EPR demonstrated the exposure of erythrocytes with fraction of human placenta extracts below 5 kDa as not affecting the microviscosity of erythrocyte cytosol. Along with that we observed the modification of temperature dependence of spin probe mobility under exposure with the same fraction of placenta extracts, stored for 6 months at –20°C. This correlated with a decrease in acid resistance of erythrocytes under the effect of this fraction.

Probl Cryobiol Cryomed 2014; 24(1):28–37.


low temperature storage; placenta extracts; erythrocytes; acid resistance; electron paramagnetic resonance

Full Text:



Arvint T., Cudd A., Shulz B. et al. Low-pH association of proteins with the membranes of intact red blood cells. Studies of the mechanism. Biochim Biophys Acta 1989; 981(1): 51–61. CrossRef

Campanella M.E., Chu H., Low P.S. Assembly and regulation of a glycolytic enzyme complex on the human erythrocyte membrane. Proc Natl Acad Sci USA 2005; 102(7): 2402–2407. CrossRef PubMed

Cheremskoy A.K., Nikitchenko Yu.V., Chizhevsky V.V. Effect of cryopreserved placenta extract on antioxidant system activity in rat tissues after general cooling. Problems of Cryobiology 2008; 18(1): 77–80.

Fujino T., Ando K., Beppu M., Kiyomi K. Enzymatic removal of oxidized protein aggregates from erythrocyte membranes. J Biochem 2000; 127(6): 1081–1086. CrossRef PubMed

Grischenko V.I. The role of cryobiology in creation of biotechno-logies for cellular and tissue transplantation. Problems of Cryobiology 2001; (3): 7–8.

Grischenko V.I., Goltsev A.N. Transplantation of products of embryofetoplacental complex. From understanding of action mechanism to increase in application efficiency. Problems of Cryobiology 2002; (1): 54–84.

Grischenko O.V., Morozova T.F., Lakhno I.V. et al. Improvement of functional properties of tissue preparations in CTHP (cryopreserved tissue of human placenta) program. Visnyk Problem Biologii i Medytsyny 1998; (19): 20–25.

Gulevsky A.K., Abakumova E.S., Moiseyeva N.N. et al. The effect of cattle cord blood fraction (below 5 kDa) on biochemical blood indices under experimental subchronic gastric ulcer in rats. Ukr. Biokhim. Zhurn. 2008; 80(2): 120–126.

Gulevsky A.K., Grischenko V.I., Relina L.I. et al. Effect of fraction below 5 kDa from brain of cold-adapted fish species on viability of Carassius auratus under cold effect conditions. Dopovidi NAN Ukrainy 2009; (11): 155–159.

Harris D.A. Spectrophotometric assays. In: Spectrophotometry & spectrofluorimetry. Washington: IRL Press; 1987: p. 49–90. PubMed

Ivanov I.T. Low pH-induced hemolysis of erythrocytes is related to the entry of the acid into cytosole and oxidative stress on cellular membranes. Biochim Biophys Acta 1999; 1415: 346–360. CrossRef

Likhtenshtein G.I. Method of spin labels in molecular biology. Moscow: Nauka; 1974.

Lutsenko N.S., Prokopyuk O.S., Bondarenko I.A. et al. Application of cryopreserved placental tissue at isoimmunization of pregnant women. Problems of Cryobiology 2008: 18(3): 316–318.

Manno S., Takakuwa Y., Mohandas N. Modulation of erythrocyte membrane mechanical function by protein 4.1 phosphorylation. J Biol Chem 2005; 280(9): 7581–7587. CrossRef PubMed

Perchik O.A. Comparative characteristics of the results of the action of cryopreserved placental extract and 'Ovestin' administered for treating urogenital disorders in women in climacteric period. Eksperiment i Klinichna Medytsyna 2006; (1): 148–150.

Pogozhikh D.N., Nardid O.A., Rozanova E.D. et al. Properties of placenta extracts derived from frozen tissues. Visnyk Problem Biologii i Medytsyny 2009; (3): 86–90.

Pogozhikh D.N., Rozanova E.D., Nardid O.A. Change in certain structural parameters of erythrocytes under the effect of placental extracts. In: Hematology and blood transfusion. Volume 33. Kiev, 2006: 139–141.

Pogozhikh D.N., Rozanova E.D., Nardid O.A. Change of properties of human placenta aqueous-saline extracts during low temperature storage. Problems of Cryobiology 2008; 18(1): 22–26.

Prokopyuk O.S., Petrenko A.Yu., Prokopyuk V.V., Chizhevsky V.V. Cryopreservation of placenta with different maturity degree. Problems of Cryobiology 2008; 18(2): 220.

Shepit'ko V.I. Effect of cryopreserved placenta preparations on peroxidative indices in patients with stenocardia. Problems of Cryobiology 2004; (1): 70–74.

DOI: https://doi.org/10.15407/cryo24.01.028


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.


Institute for Problems of Cryobiology and Cryomedicine

23, Pereyaslavskaya str., Kharkov, Ukraine

Tel. +38057 373 4143; Fax +38057 373 5952

e-mail: journal@cryo.org.ua