Effect of Hormone Injections on Quality Parameters and Cryoresistance of Sperm from Silver Carp (Hypophthalmichthys molitrix, Val. 1844)

Authors

  • Kyrylo I. Butskyi Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Anton Yu. Puhovkin Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Eugeniy F. Kopeika Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

DOI:

https://doi.org/10.15407/cryo24.02.140

Keywords:

sperm, silver carp, hormone stimulation, cryopreservation, ATP, sperm concentration

Abstract

The influence of different hormonal stimulation of final stages of gametogenesis in silver carp (Hypophthalmichthys molitrix, Val. 1844) on the quality of its sperm was studied. Preparations were used in following dosage: pituitary – 2.5 mg/kg; Surfagon – 1 mg/kg combined with Metoclopramide – 5 mg/kg; Ovopel – 0.5 pellet/kg. It was found that after stimulation of sires with a mixture of Surfagon and Metoclopramide the cell concentration in semen was higher than after stimulation by drug Ovopel or carp pituitary, and it was (9.7 ± 0.9); (10.7 ± 0.8); (13.3 ± 0.9) bln/ml, correspondingly. The ATP concentration in sperm after stimulation by this drug also was almost twice bigger and it was (51.9 ± 2.1) nmol/mg protein, and in case of Ovopel and carp pituitary it was (18.5 ± 0.7) and (30.3 ± 4) nmol/mg protein. A higher concentration of active motile spermatozoa after cryopreservation may indicate a relatively high quality of the sperm.


Probl Cryobiol Cryomed 2014; 24(2):140-148.

Author Biographies

Kyrylo I. Butskyi, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiology of Reproduction System

Anton Yu. Puhovkin, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiology of Reproduction System

Eugeniy F. Kopeika, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiology of Reproduction System

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Published

2014-06-20

How to Cite

Butskyi, K. I., Puhovkin, A. Y., & Kopeika, E. F. (2014). Effect of Hormone Injections on Quality Parameters and Cryoresistance of Sperm from Silver Carp (Hypophthalmichthys molitrix, Val. 1844). Problems of Cryobiology and Cryomedicine, 24(2), 140–148. https://doi.org/10.15407/cryo24.02.140

Issue

Vol. 24 No. 2 (2014): Problems of Cryobiology and Cryomedicine

Section

Theoretical and Experimental Cryobiology

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Copyright (c) 2020 Kyrylo I. Butskyi, Anton Yu. Puhovkin, Eugeniy F. Kopeika

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