New Fluorescent Dyes as Fluorescent Probes for Examination of Canine Spermatozoa in Cryoprotective Media
Keywords:canine spermatozoa, dimethyl formamide, ethylene glycol, fluorescent probes, DSM, E-176, 3-DAB, FME
Possibilities of using DSM, E-176, 3-DAB, FME fluorescent dyes for examining the effect of cryoprotective agents on canine spermatozoa were studied with the methods of fluorescent spectroscopy and microscopy. FME and 3-DAB dyes were found to be capable of solving the tasks set and DSM, E-176 ones had a limitation due to quite a strong fluorescence of cryoprotectantsâ€™ solutions. Studied fluorescent dyes were demonstrated as differently affecting cell motility.
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Copyright (c) 2020 M. I. Еgorov, T. S. Dyubko, T. P. Linnik, A. P. Belonozhko, I. G. Еrmolenko, O. N. Semenova, L. D. Patsenker, V. G. Pivovarenko, E. A. Povrozin
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