Effect of Penetrating Cryoprotectants and Low Temperature on Conformational State of Microsomal Proteins on UV-Spectrophotometry Data

Authors

  • E. V. Onischenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. S. Dyubko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

microsomes, proteins, cryoprotectants, glycerol, 1, 2- propane diol, dimethylsulfoxide, freezing, spectrophotometry

Abstract

Effect of penetrating low molecular cryoprotectants (CPs): glycerol, 1,2-propanediol (1,2-PD) and dimethyl sulfoxide (DMSO) as well the one of freeze-thawing with these cryoprotectants on conformational state of microsomal membrane proteins (MMP) derived from rats’ liver have been studied with UV-spectrophotometry. The efficiency of CPs interaction with MMP has been shown to depend both on their concentration and individual physical and chemical properties. The degree of CPs effect on MMP during freeze-thawing in greater extent depends on pre-sensibilization of microsomal membranes at the stage of their equilibration with CPs. Examined CPs on ascending of perturbing effect on MMP state could be disposed in the row: glycerol < 1,2 PD < DMSO.

Downloads

Published

2006-06-15

How to Cite

Onischenko, E. V., & Dyubko, T. S. (2006). Effect of Penetrating Cryoprotectants and Low Temperature on Conformational State of Microsomal Proteins on UV-Spectrophotometry Data. Problems of Cryobiology and Cryomedicine, 15(2), 184–194. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/631

Issue

Vol. 15 No. 2 (2005): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 E. V. Onischenko, T. S. Dyubko

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).