Study of Effect of Some Permeable Cryoprotectants on Microsomes Using Chemiluminescence Method
Keywords:liver microsomes, cryoprotectants, lipid peroxidation, free-radical processes, chemiluminescence
The effect of freezing and low molecular cryoprotectants on the intensity of peroxidative processes in ratâ€™s liver microsomes have been studied using chemiluminescence (CL) method. As cryoprotectants (CP) we used solutions of glycerol (Gl), 1,2-propane diol (1,2-PD) and DMSO, prepared on Tris-buffer within the concentration range from 5 to 40% (w/w). The values of spontaneous chemiluminescence (SCL), amplitude, H2O2 light sum and Fe2+-induced CL were noted to augment when the cryoprotectant content in microsome suspension was higher than 30% (w/w).
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Copyright (c) 2020 E. V. Onischenko, A. V. Zinchenko
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