Effect of Glycerol, Ethylene Glycol on Morphofunctional Characteristics of Dogs’ Spermatozoa at Different Cryopreservation Stages

Authors

  • M. I. Egorov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. F. Kopeika Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. P. Linnik Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. N. Kuchkov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

spermatozoa, motility, equilibration, cryopreservation, glycerol, ethylene glycol

Abstract

There has been studied the effect of glycerol and ethylene glycol (EG) on survival of dogs’ spermatozoa (DS) after freeze-thawing depending on the concentration of cryoprotectants, cooling regimens and equilibration time, as well as the change in DS cell volume after the cryoprotectants being introduced into suspension by means of turbidimetric method. The highest motility of DS was revealed after cryopreservation under EG protection with final concentration of 7% when being frozen at the first stage with the rate of 3°C/min with no preliminary equilibration. It has been shown that 7% EG causes quite less alterations in cell volume if compared to glycerol.

Published

2004-12-15

How to Cite

Egorov, M. I., Kopeika, E. F., Linnik, T. P., & Kuchkov, I. N. (2004). Effect of Glycerol, Ethylene Glycol on Morphofunctional Characteristics of Dogs’ Spermatozoa at Different Cryopreservation Stages. Problems of Cryobiology and Cryomedicine, (4), 13-20. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/655

Issue

Section

Theoretical and Experimental Cryobiology