Forecasting of Murine Embryo Osmotic Behaviour at Main Stages of Cryopreservation

Authors

  • O. V. Pishko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. I. Smolyaninova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. F. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • S. E. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. F. Rozanov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

embryo, ethylene glycol, osmotic reaction, permeability coefficient

Abstract

There were determined the transport characteristics for zygotes, 2- and 8-cell murine embryos. Basing on the obtained permeability coefficients and corresponding geometric cell parameters the modelling of cell osmotic behaviour at the stages of cryoprotectant adding to embryos, as well as at the stages of embryo freezing with different cooling rates and cryoprotectant removal out of cells, was carried-out.

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Published

2004-09-15

How to Cite

Pishko, O. V., Smolyaninova, E. I., Kovalenko, I. F., Kovalenko, S. E., & Rozanov, L. F. (2004). Forecasting of Murine Embryo Osmotic Behaviour at Main Stages of Cryopreservation. Problems of Cryobiology and Cryomedicine, (3), 3–8. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/668

Issue

No. 3 (2004): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 O. V. Pishko, E. I. Smolyaninova, I. F. Kovalenko, S. E. Kovalenko, L. F. Rozanov

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