Cryoprotective Properties of Solutions Based on Non-Penetrative OEGn = 25 Combined with Penetrating Cryoprotectants During Freezing of Human Erythrocytes

Authors

  • Yuliya S. Pakhomova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Valentina V. Chekanova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Antonina M. Kompaniets Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

red blood cells, cryopreservation, combined cryoprotective solutions, oxyethylated glycerol

Abstract

Cryoprotective efficiency of сryopreservatives, based on combination of extracelular cryoprotectant oxyethylated glycerol with polymerization degree n = 25 (OEGn=25) with penetrating cryoprotectants 1,2-propanediol (1,2-PD), dimethyl sulphoxide (DMSO) and dimethyl acetamide (DMAc) during red blood cells freezing was studied. Freeze-thawing in solutions of OEGn=25 and 1,2-PD, DMSO and DMAc mixed in 5:1 ratio, as well as with DMAc in ratios of 2:1 and 1:1 resulted in the reduction of osmotic fragility of red blood cells comparing to non-treated cells. The highest level of post-thaw survival of red blood cells according to indices of hemolysis and content of free hemoglobin was found for 30% OEGn=25 solution. Probl Cryobiol Cryomed 2013; 23(1):26–39.

Author Biographies

Yuliya S. Pakhomova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryoprotectants

Valentina V. Chekanova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryoprotectants

Antonina M. Kompaniets, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryoprotectants

References

Agranenko V.A., Fedorova L.I. Frozen blood and its clinical application Moscow: Medicine, 1983.

Babijchuk L.A. Conformational changes in erythrocytes under the effect of PEO–1500 cryoprotectant. Problems of Cryobiology 1997; (1–2): 95–99.

Babijchuk L.A., Zemlyanskikh N.G. Optimization and advantanges of washing-out method for erythrocytes cryopreservation with PEO-1500. Problems of Cryobiology 2001; (1): 35–41.

Bagautdinov S.M. Improving of long-term storage of blood and bone marrow in a frozen state in Blood transfusion services of the Armed Forces [Author’s abstract of thesis ... Doctor of Biol. Sciences]. St. Petersburg, 2000.

Belous A.M., Bondarenko V.A. Structural changes in biological membranes during cooling Kiev: Nauk. Dumka, 1982.

Vinograd-Finkel F.R., Leontovich V.A. Theoretical problems of cryopreserved blood cells. Problemy Gematologii i Perelivaniya Krovi 1977; 22 (2): 3–9.

Vinograd-Finkel F.R., Fedorova L.I., Semenova N.V. Valuability of red blood cells, stored during 12–16 years at –196°C. Problemy Gematologii i Perelivaniya Krovi 1980; 25 (1): 8–11.

Gordienko O.I., Gordienko E.A., Linnik T.P., Kompaniets A.M. Mechanisms of cryoprotectant permeation via erythrocytes membranes. Problems of Cryobiology 2002; (4): 9–15.

Yesipova Yu.S., Nikolenko A.V., Chekanova V.V., Kompaniets A.M. Influence of ethoxylated derivatives of glycerol on the stability of red blood cells to the action of hypertonic stress. Proceedings of the VII International Scientific and Technical Conference ‘Actual Problems of Biological Physics and Chemistry. BFFH’ 2011: 76–77.

Zhivotova E.N., Zinchenko A.V., Chekanova V.V., Kompa-niets A.M. Thermal analysis of binary systems of water – ethoxylated glycerol (degree of polymerization n = 5 and 25) at temperatures below 273 K. Dopovidi of National Academy of Sciences of Ukraine 2006; (9): 74–79.

Zhivotova E.N., Kuleshova L.G., Zinchenko A.V., Chekanova V.V. Investigation of the physical states of the binary systems of water – glycerol ethoxylated with a degree of polymerization of n = 5 and 25 at temperatures below 273 K using cryomicroscopyi. Dopovidi of the National Academy of Sciences of Ukraine 2007; (4): 78–84.

Kozlov A.A., Prostakova T.M., Berkovskiy A.L. Manual for laboratory doctors for assessing hemoglobin content Moscow, 2006.

Kompaniets A.M., Nikolenko A.V., Chekanova V.V., Trots Yu.P. Cryopreservation of red blood cells under the protection of the ethoxylated glycerol oligomer. Problems of Cryobiology2005; 15 (3): 561–565.

Kompaniets A.M., Nikolenko O.V., Chekanova V.V., Yesipova Yu.S. Cryoprotective efficiency of media based on oxyethyl derivatives of polyols during freezing of human erythrocytes. Problems of Cryobiology 2008;18 (3): 299–301.

Loevskiy M.M. Effect of glycerol (propane triol) and 1,2-propane diol on the structural and functional characteristics of cryopreserved erythrocytes [Author’s abstract of thesis ... Candidate Biol. Sciences Kharkov]. 1984.

Lubyanyi V.G., Bredikhina L.P., Shrago M.I. Cryoprotective activity of OEG oligomers in the red cell low temperature preservation. Kriobiologiya i Kriomeditsina 1981 Issue 8: 34–40.

Martynenko A.A., Lugovoy V.I. Effect of PEO-1500 and low temperatures on the isoosmotic resistance of human erythrocytes. Kriobiologiya i Kriomeditsina 1989; (8): 13–16.

Mezhidov S.H., Moiseyev V.A., Nardid O.A. Dehydration of erythrocyte caused by components of cryopreservation media. Kriobiologiya i Kriomeditsina 1989; (2): 13–16.

Menshikov V.V. Laboratory methods of investigation in the clinic: Methods of hematological studies Moscow: Meditsina, 1987.

Nіkolenko O.V., Yesіpova Yu.S., Kompanіyets A.M. Cryopreservation of human erythrocytes under protection of polymer homologue of oxyethylated glycerol (OEGn = 30). Gematologіya i Perelyvannya Krovі 2008; 34 (2): 166–170.

Ramazanov V.V., Bondarenko V.A. Osmotic properties of erythrocytes frozen in media containing non-penetrating and penetrating cryoprotectants. Problems of Cryobiology 2010; 20 (1): 47–57.

Consolidated List of Products Whose Consumption and/or Sale Have Been Banned, Withdrawn, Severely Restricted or Not Approved by Governments. Pharmaceuticals New York, 1996.

Khaylachev E.E. Optimization of cryopreservation programs for backup of hemotherapeutical means [Author’s abstract of thesis ... Candidate Med. Sciences]. St. Petersburg, 2009.

Shrago M.I., Guchok M.M., Kalugin Yu.V. Some principles of direct synthesis of cryoprotectants. In: Current Problems of Cryobiology / Eds. N.S. Pushkar and A.M. Belous. Kiev: Naukova Dumka, 1981: 157–201.

Chaudhari. S. Frozen red blood cells transfusion. MJAFI 2009; 65 (1): 55–58.

Council of Europe guide to the preparation, use and quality assurance of blood components. Recommendation R (95) 15, 11th Strasbourg: Council of Europe, 2005.

Fuller B.J. Cryoprotectants: The essential antifreezes to protect life in the frozen state. CryoLetters 2004; 25 (6): 375–388.

Gurtovenko A.A., Anwar J. Modulating the structure and properties of cell membranes: the molecular mechanism of action of dimethyl sulfoxide. J Phys Chem B 2007; 111 (33): 10453–10460.

Holovati J.L., Gyongyossy-Issa M.I., Acker J.P. Effect of tregalose-loaded liposomes on red blood cell response to freezing and post-thaw membrane quality. Cryobiology 2009; 58 (2): 75–83.

Huggins C.E. Prevention of hemolysis of large volumes of red blood cells slowly frozen and thawed in the presence of dimethylsulfoxide. Transfusion 1963; 3 (1): 483–493.

Lynch A.L., Chen R., Dominowski P.J. et al. Biopolymer mediated trehalose uptake for enhanced erythrocyte cryosurvival. Biomaterials 2010; 31 (23): 6096–6103.

Pribor D.B. Osmotic рemolysis contrasted with freeze–thaw hemolysis. Cryobiology 1971; 8 (1): 14–24.

Quan G., Zhang B. L., Guo Y. et al. Intracellular sugars improve survival of human red blood cells cryopreservation at –80°C in the presence of polyvinylpyrrolidone and human serum albumin. CryoLetters 2008; 28 (2): 95–108.

Singbartl K., Langer R., Henrich A. Altered membrane skeleton of hydroxyethylstarh–cryopreserved human erythrocytes. Cryobiology 1998; 36 (2): 115–123.

Sputtek A., Langer R., Singbartl G. Cryopreservation of red blood cells with the non-penetrating cryoprotectant hydroxyethyl starh. CryoLetters 1995; 16 (4): 283–288.

Sputtek A., Kuehnl P., Rowe A.W. Cryopreservation of erythrocytes, thrombocytes and lymphocytes. Transfus Med Hemother 2007; 34 (4): 262–267.

Sputtek A., Rowe A.W. Looking back from the future to the present: biopreservation will get us there. Transfus. Med. Hemother 2011; 38 (1): 85–87.

Stoll Ch., Wolkers W.F. Membrane stability during biopreservation of blood cells. Transfus Med Hemother 2011;38 (1): 89–97.

Sum A.K., Pablo J.J. Molecular study on the influence of dimethylsulfoxide on the structure of phospholipids bilayers. Biophys J 2003; 85 (6): 3636–3645.

Takahashi T., Hirsh A., Williams R.J. Mechanism of cryoprotection by extracellular polymeric solution. Biophys J 1988; 54 (3): 509–518.

Thomas M.J.G. The long term storage of blood cells. Current problems of transfusion medicine in clinical practice. Ed. by A.A.M. Todd, U. Rossi Milano: ESTM, 1993: 117–120.

Thomas M. J.G., Parry E.S, Nash S.G., Bell S.H. A method for the cryopreservation of red blood cells using hydroxyethyl starch as a cryoprotectant . Transfusion Science 1996; 17 (3): 385–396.

Valery C.R., Ragno G., Van Houten P. et al. Automation of the glycerolization of red blood cells with high-separation bowl in Haemonetics ACP 215 instruments. Transfusion 2005; 45 (3): 1621–1627.

Published

2013-03-15

How to Cite

Pakhomova, Y. S., Chekanova, V. V., & Kompaniets, A. M. (2013). Cryoprotective Properties of Solutions Based on Non-Penetrative OEGn = 25 Combined with Penetrating Cryoprotectants During Freezing of Human Erythrocytes. Problems of Cryobiology and Cryomedicine, 22(1), 26-39. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/7

Issue

Section

Theoretical and Experimental Cryobiology