Effect of Cryoprotectants on Hypertonic Cryohemolysis Rate of Human Erythrocytes with Modified Cytoskeleton-Membrane Complex
Keywords:hypertonic cryohemolysis, erythrocytes, cryoprotectants, modifiers of protein sulfhydryl groups
There was studied the development of hypertonic cryohemolysis of erythrocytes, modified with iodoacetamide (IAA) and pchlormercuribenzoate (p-CMB) in the media containing cryoprotectants (glycerol, 1,2-propane diol, polyethylene glycerol-1500). It has been shown that independently on the presence in the incubation medium of cryoprotective compounds when modifying the protein component of erythrocytes with IAA and p-CMB the sensitivity of erythrocytes to hypertonic cryohemolysis increases. This may be stipulated with the change in conformation of the proteins directly or indirectly involved into adaptation mechanisms of cells to stress effects.
How to Cite
Copyright (c) 2020 Ya. O. Cherkashina
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).