Parameters of Spectral Analysis of Heart Rate Variability in Rats

Nikolay A. Chizh

Abstract


The paper considers the synthesis and analysis of the reported data on the use of the parameters of spectral analysis when studying the heart rate variability (HRV) in rats. There were demonstrated the criteria for electrocardiographic equipment to perform studies in small mammals, substantiated the indices of duration of electrocardiogram (ECG) recording and indicated the necessity in stationary state of animals during ECG recording. When describing the ranges of spectral frequencies and data-based reports we suggested the parameters of HRV spectral analysis during 5-minute ECG recording in conscious rats such as: TP – 0.015–3 Hz; VLF – 0.015–0.04 Hz; LF – 0.05–0.79 Hz; HF – 0.8–3 Hz. The paper pointed to the need of performing extra-studies on the Mayer wave frequency, as well as the expediency of HRV spectral analysis together with respiration diagram recording. To clarify the parameters of spectral frequencies we recommended the findings comparing with the results of other research methods of HRV. The data of statistical processing of cardiointervalograms during ECG recording in unrestrained rats will enable to specify the selected parameters, accept a common protocol of HRV spectral analysis in this animal species and evaluate their vegetative status. Probl Cryobiol Cryomed 2015; 25(3):235-245.


Keywords


heart rate variability; spectral analysis; frequency ranges; rats

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Albarwani S., Al-Siyabi S., Tanira M.O. Lisinoprl indifferently improves heart rate variability during day and night periods in spontaneously hypertensive rats. Physiol Res 2013; 62 (3): 237–245. PubMed

Alipov N.N., Sergeeva O.V., Kuznetsova T.E. et al. Role of sympathetic and parasympathetic nervous systems in heart rate regulation in cats. Bull Exp Biol Med 2005; 140 (11): 484–489. CrossRef

Baevsky R.M. Cybernetic analysis of managerial processes of a heart rhythm. In: Actual problems of physiology and blood circulation pathology.Vol. 59. Moscow: Meditsina; 1976; P. 161–175.

Baevsky R.M., Ivanov G.G., Chireykin L.V. et al. Analysis of heart rate variability using different electrocardiographic systems. Vestnik Aritmologii 2001; (24): 65–87.

Baevsky R.M., Kirillov O.I., Kletskin S.Z. Mathematical analysis of heart rate changes under stress. Мoscow: Nauka; 1984.

Baevsky R.M., Nidekker I.G. Methods and some results of autocorrelation and spectral analysis of heart rate. In: Computing techniques in physiology and medicine. Moscow; 1968; P. 151–162.

Ban A.S., Paramonova N.A., Zagorodnyy G.M., Ban D.S. Analysis of the relationship of heart rate variability indices. Voennaya Meditsina 2010; (4): 21–24.

Barres C., de Souza Neto E.P, Julien C. Effect of alphaadrenoceptor blockade on the 0.4 Hz sympathetic rhythm in conscious rats. Clin Exp Pharmacol Physiol 2001; 28 (12): 983–985. CrossRef PubMed

Baumert M., Brechtel L. M., Lock J. et al. Scaling graphs of heart rate time series in athletes demonstrating the VLF, LF and HF regions. Physiol Meas 2006; 27 (9): 35–39. CrossRef PubMed

Bertram D., Orea V., Chapuis B. et al. Differential responses of frequency components of renal sympathetic nerve activity to arterial pressure changes in conscious rats. Am J Physiol Regul Integr Comp Physiol 2005; 289 (4): 1074–1082. CrossRef PubMed

Blanc J., Grichois M.L., Vincent M. et al. Spectral analysis of blood pressure and heart rate variability in response to stress from air-jet in the Lyon rat. J Auton Pharmacol 1994; 14 (1): 37–48. CrossRef PubMed

Bosquet L., Merkari S., Arvisais D. et al. Is heart rate a convenient tool to monitor over-reaching? A systematic review of the literature. Br J Sports Med 2008; 42 (9): 709–714. CrossRef PubMed

Brown D.R., Brown L.D., Patwardhan A. et al. Sympathetic activity and blood pressure are tightly coupled at 0.4 Hz in conscious rats. Am J Physiol 1994; 267 (5): 1378–1384.

Carnevali L., Sgoifo A. Vagal modulation of resting heart rate in rats: the role of stress, psychosocial factors, and physical exercise. Front Physiol 2014; 24 (5): 1–10. CrossRef

Cerutti C., Barres C., Paultre C. Baroreflex modulation of blood pressure and heart rate variabilities in rats: assessment by spectral analysis. Am J Physiol 1994; 266 (5): 1993–2000.

Chen S.W. A new algorithm developed bases on a mixture of spectral and nonlinear techniques for the analysis of heart rate variability. J Med Eng Technol 2007; 31 (3): 210–219. CrossRef PubMed

Cheng Y., Cohen B., Orea V. et al. Baroreflex control of renal sympathetic nerve activity and spontaneous rhythms at Mayer wave's frequency in rats. Auton Neurosci 2004; 111 (2): 80–88. CrossRef PubMed

Chizh N.A., Babaeva A.G, Galchenko S.E., Sandomirskiy B.P. Heart rate variability when modeling myocardial necrosis by surgical method. Exp Clin Med 2011; 51 (2): 44–48.

Daffonchio A., Franzelli С., Radaelli А. et al. Sympathectomy and cardiovascular spectral components in conscious normotensive rats. Hypertension 1995; 25 (6): 1287–1293. CrossRef PubMed

Dobrek L., Skowron B., Baranowska A., Thor P.J. Spectral heart rate variability analysis in experimental obstructive and chemical overactive bladder models. Adv Clin Exp Med 2013; 22 (3): 337–346. PubMed

Durnova N.Yu., Dovgalevskiy Ya.P., Burlaka A.N. et al. Interdependence of parameters of variational pulsometry, entropy of heart rate, temporal and spectral analyses of heart rate variability in normal state and in ischemic heart disease. Saratov Journal of Medical Scientific Research 2011; 7 (3): 607–611.

Dvornikov A.V., Mukhina I.V., Krylov V.N. Changes in heart rate variability under emotional stress conditions in rats with administration of adrenergic blocker of b1-receptors. Nizhegorod Med Zhurnal 2003; (1): 8–12.

Haspekova N.B. Diagnostic informative monitoring of heart rate variability. Vestnik Aritmologii 2003; 32: 15–23.

Hauton D., May S., Egginton S. et al. Cold-impaired cardiac performance in rats is only partially overcome by cold acclimation. J Exp Biol 2011; 214: 3021–3031. PubMed

Heart rate variability: Standards of measurement, physiological interpretation, and clinical use. Task Force of the European Society of Cardiology and the North American Society of Pacing and Electrophysiology. Circulation 1996; 93 (5): 1043–1065. CrossRef PubMed

Ivanov A.P., Fateyev D.M. Changes of heart rate variability of rats after kidney resection. Yaroslavl Ped Bull 2011; 3 (3): 94–97.

Julien C. The enigma of Mayer waves: facts and models. 2006; 70 (1): 12–21.

Kaverina N.V., Popova E.P. Influence of an III class antiarrhythmic cardiocyclid on heart rate variability in rats under emotional stress. Vestnik Aritmologii 2010; 62: 47–51.

Kaverina N.V., Popova E.P., Yarkova M.Ya., Seredenin S.B. Aphobazolum effect on heart rate variability in rats with different behaviors in ‘open field’ test. Exp Klin Farmakol 2009; 72 (1): 33–40. PubMed

Korkushko O.V., Pisaruk A.V., Shatilo V.B. et al. Analysis of heart rate variability in clinical practice (age-sensitive). Kiev; 2002.

Kornelyuk I.V., Nikitin Ya.G., Koptyukh T.M. et al. Significance of heart rate variability assessment to evaluate the efficiency of differentiated anti–arrhythmic therapy of atrial fibrillation. Vestnik Aritmologii 2004; 36: 18–22.

Korolev I.B., Kotelnikov V.N., Osipov I.O. Changes in spectral parameters of heart rate variability in rats with elevated blood pressure. Zdorovie Med Ekologiya Nauka 2014; 56 (2): 38–41.

Korolev I.B., Kotelnikov V.N., Osipov I.O. Comparative assessment of state of sympathetic and parasympathetic parts of autonomic nervous system at high blood pressure level in rats. Science and World 2014; 7 (3): 122–127.

Kruger C., Kalenka A., Haunstetter A. et al. Baroreflex sensitivity and heart rate variability in conscious rats with myocardial infarction. Am J Physiol 1997; 273 (5): 2240–2247.

Kumae T. Assessment of training effects on autonomic modulation of the cardiovascular system in mature rats using power spectral analysis of heart rate variability. Environ Health Prev Med 2012; 17 (5): 415–422. CrossRef PubMed

Kumae T., Arakawa H. A study for assessment of day-night cycle disturbance effects on the autonomic modulation in freely behaving rats using spectral analysis of heart rate variability. J Phys Fit Neut Immun 2003; 13: 112–120.

Kuryanova E.V. Vegetative regulation of cardiac rhythm: results and prospects of researh. Astrakhan: Publishing House ‘Astrakhanskiy Universitet’; 2011.

Kuryanova E.V., Teplyy D.L. Features of reaction types to a sharp stress by the parameters of heart rate variability in nonlinear rats against administration of alpha tocopherol. Proceeding of the Samara Scientific Center of Rus Acad Sci 2012; 14 (5): 471–475.

Kuryanova E.V., Tepliy D.L. Influence of central neurotransmitters on heart rate variability in outbred rats at rest and during acute stress: to the question on the nature of verylow-wave spectrum component. Bull Exp Biol Med 2010; 149 (1): 14–17.

Kuwahara M., Yayou K., Ishii K. et al. Power spectral analysis of heart rate variability as a new method for assessing autonomic activity in the rat. J Electrocardiol 1994; 27(4): 333–337. CrossRef

Lloyd E., Lederman U., Aivazyan S.A., Tyurin Yu.N., editors. Applied statistics handbook. Vol. 2. Moscow: Finansy i statistika; 1990.

Lutsenko D.G., Shylo A.V., Marchenko L.N. et al. Peculiarities of heart rhythm regulation at different types of cold acclimation in rats. Problems of Cryobiology and Cryomedicine 2013; 23 (2): 105–115.

Malliani A., Pagani M., Lombardi F. et al. Cardiovascular neural regulation explored in the frequency domain. Circulation 1991; 84 (2): 482–492. CrossRef PubMed

Marocolo M., Maior A., Katayama P. et al. Anabolic steroid treatment induces cardiac autonomic dysfunction in rats: timecourse of heart rate variability. Am J Biomed Eng 2013; 3 (3): 54–62.

Mashin V.A. Nonstationarity and duration of the cardiac interval time series in assessing the functional state of personnel. Biofizika 2007; 52(2): 344–354. CrossRef

Mazloom R., Eftekhari G., Rahimi M. et al. The Role of α7 nicotinic acetylcholine receptor in modulation of heart rate dynamics in endotoxemic rats. PLoS One 2013; 8 (12): e82251. CrossRef PubMed

Mikhailov V.M. Heart rate variability: experience of practical application of the method. Ivanovo; 2002.

Montano N., Ruscone T., Porta A. et al. Power spectrum analysis of heart rate variability to assess the changes in sympathovagal balance during graded orthostatic tilt. Circulation 1994; 90 (4): 1826–1831. CrossRef PubMed

Nozdrachev A.D. Physiology of the autonomic nervous system. Leningrad; 1983.

Ori Z., Monir G., Weiss J. et al. Heart rate variability. Frequency domain analysis. Cardiol Clin 1992; 10 (3): 499–537. PMid:1504981

Pereira-Junior P.P., Chaves E.A., Costa-e-Sousa R.H. et al. Cardiac autonomic dysfunction in rats chronically treated with anabolic steroid. Eur J Appl Physiol 2006; 96: 487–494. CrossRef PubMed

Pereira-Junior P.P., Marocolo M., Rodrigues F.P. et al. Noninvasive method for electrocardiogram recording in conscious rats: feasibility for heart rate variability analysis. An Acad Bras Sci 2010: 82 (2): 431–437. CrossRef

Pokrovskii V.M. Hierarchy of the heart rhythmogenesis levels is a factor in increasing the reliability of cardiac activity. Med Hypotheses 2006; 66 (1): 158–164. CrossRef PubMed

Ramaekers D., Beckers F., Demeulemeester H., Aubert A. Cardiovascular autonomic function in conscious rats: a novel approach to facilitate stationary conditions. Ann Noninvasive Electrocardiol 2002; 7: 307–318. CrossRef PubMed

Ryabykina G.V., Sobolev A.V. Heart rate variability. Moscow: StarKo; 1998.

Sabharwal R., Coote J.H., Johns E.J., Egginton, S. Effect of hypothermia on baroreflex control of heart rate and renal sympathetic nerve activity in anaesthetized rats. J Physiol 2004; 557: 247–259. CrossRef PubMed

Salnikov, E.V., Fateev M.M., Sidorov A.V. et al. Effect of betaadrenoblockers on heart rate variability in conscious and narcotized rats. Bull Exp Biol Med 2007; 144 (10): 372–375.

Sergeeva O.V., Alipov N.N., Smirnov V.M. Effect of atropine, propranolol and atenolol on the wave structure of heart rate oscillations in rats. Bull Exp Biol Med 2008; 145 (5): 364–367. CrossRef

Silveira L.C.R., Tezini G.C., Schujmann S.D. et al. Comparison of the effects of aerobic and resistance training on cardiac autonomic adaptations in ovariectomized rats. Autonomic Neuroscience: Basic and Clinical 2011; 162: 35–41. CrossRef PubMed

Sitdikov F.G., Gil'mutdinova R.I., Minnakhmetov R.R., Zefirov T.L. Asymmetrical effects of vague nerves on functional parameters of rat heart in postnatal ontogenesis. Bull Exp Biol Med 2000; 130 (7): 10–13.

Thayer J.F., Yamamoto S.S., Brosschot J.F. The relationship of autonomic imbalance, heart rate variability and cardiovascular disease risk factors. Int J Cardiol 2010; 141 (2): 122–131. CrossRef PubMed

Xin-Yan G., Kun L., Bing Z., Litscher G. Sino-European transcontinental basic and clinical high-tech acupuncture studies – Part 1: Auricular acupuncture increases heart rate variability in anesthetized rats. Evid Based Complement Alternat Med 2012: 8173–8178.

Yabluchansky N.I., Martynenko A.V. Heart rate variability to help practicing physician. Kharkov; 2010.

Yukinaka M., Nomura M., Saijyo T. et al. Evaluation of autonomic nervous function in patients with essential hypertension complicated with peptic ulcer. J Gastroenterol Hepatol 2000; 15 (1): 40–44. CrossRef PubMed

Zapadnyuk I.P., Zapadnyuk V.I., Zakharia E.A., Zapadnyuk B.V. Laboratory animals. Breeding, maintenance, use in experiments. Kiev: Vyscha shkola; 1983.

Zarubin F.E. Heart rate variability: measurement standards, indices, method peculiarities. Vestnik Aritmologii 1998; (10): 25–30.

Zigon N.J. Blood pressure and heart rate spectral changes induced by the modulation of the cholinergic transmission by physostigmine and neostigmine. Jugoslav Physiol Pharmacol Acta 1998; 34 (1): 111–120.




DOI: https://doi.org/10.15407/cryo25.03.235

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