Ultrastructure of Porcine Pericardium and Aortic Valve Tissues, Devitalized with Cryogenic and Irradiation Exposures

Authors

  • Nikolay V. Repin Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Larisa N. Marchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Tatiana P. Govorukha Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Irina P. Mikhailova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Anna A. Manchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Boris P. Sandomirsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

DOI:

https://doi.org/10.15407/cryo25.03.246

Keywords:

acellular xenogeneic materials, devitalization, freeze-thawing, ionizing irradiation, ultrastructure, pericardium, aortic valve

Abstract

This paper deals with morphological assessment of cryo- and radiodevitalized tissue biomaterial, i. e. pericardium and aortic valve as well as a cell-free xeno-carcass designing. Structure of the studied tissues after low temperature, ionizing and combined exposures was investigated by optical and electron microscopies. The application of this method to devitalize the pericardium and aortic valve is shown to result in the expressed destructive changes of cell elements, but the spatial location and structural integrity of connective tissue fibrous components are preserved.


Probl Cryobiol Cryomed 2015; 25(3):246-254.

Author Biographies

Nikolay V. Repin, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryomorphology

Larisa N. Marchenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryomorphology

Tatiana P. Govorukha, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryomorphology

Irina P. Mikhailova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Experimental Cryomedicine

Anna A. Manchenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Experimental Cryomedicine

Boris P. Sandomirsky, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Experimental Cryomedicine

References

Byzov D.V., Chizh N.A., Mikhaylova I.P. et al. Devitalized vascular prostheses: in vivo study. Vestnik Transplantologii i Iskusstvennykh Organov 2011; 12 (4): 81–90.

Conklin B., Richter E., Kreutziger K. et al. Development and evaluation of a novel decellularized vascular xenograft. Med Eng & Physics 2002; 24 (3): 173–183. CrossRef

Grant R.A., Cox R.W., Kent C.M. The effects of irradiation with high energy electrons on the structure and reactivity of native and cross-linked collagen fibres. J Cell Sci 1970; 7 (2): 387–405. PubMed

Grassl E.D., Barocas V. Effects of freezing on the mechanical properties of blood vessels. ASME Heat Transfer Division HTD 2004; 375: 699–703. CrossRef

Gratzer P., Harrison R., Woods T. Matrix alteration and not residual sodium dodecyl sulfate cytotoxicity affects the cellular repopulation of a decellularized matrix. Tissue Eng 2006; 12 (10): 2975–2983. CrossRef PubMed

Muratov R., Britikoc D., Sachkov A. et al. New approach to reduce allograft tissue immunogenicity. Experimental data. Cardio Vasc Thorac Surg 2010; 10 (3): 408–412. CrossRef PubMed

Ravi S., Chaikof E.L. Biomaterials for vascular tissue engineering. Regen Med 2010; 5 (1): 107–120. CrossRef PubMed

Schoen F., Levy R. Calcification of tissue heart valve substitutes: progress toward understanding and prevention. Ann Thorac Surg 2005; 79 (3): 1072–1080. CrossRef PubMed

Seto A., Gatt J.R. Improved tendon radioprotection by combined cross-linking and free radical scavenging. Clin Orthop Relat Res 2009; 467 (11): 2994–3001. CrossRef PubMed

Teebken O.E., Haverich A. Tissue Engineering of Small Diameter Vascular Grafts. Eur J Vasc Endovasc Surg 2002; 23: 475–485. CrossRef

Valentin J., Stewart-Akers A., Gilbert T. et al. Macrophage participation in the degradation and remodeling of extracellular matrix scaffolds. Tissue Eng 2009; 15 (7), Pt. A.: 1687–1694.

Yang J., Yamato M., Shimizu T. et al. Reconstruction of functional tissues with cell sheet engineering. Biomaterials 2007; 28 (34): 5033–5043. CrossRef PubMed

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Published

2015-09-21

How to Cite

Repin, N. V., Marchenko, L. N., Govorukha, T. P., Mikhailova, I. P., Manchenko, A. A., & Sandomirsky, B. P. (2015). Ultrastructure of Porcine Pericardium and Aortic Valve Tissues, Devitalized with Cryogenic and Irradiation Exposures. Problems of Cryobiology and Cryomedicine, 25(3), 246–254. https://doi.org/10.15407/cryo25.03.246

Issue

Section

Theoretical and Experimental Cryobiology