Influence of Autoclaved Placenta Extracts on Phase Behavior of Cell Suspensions Below 0°C

Authors

  • Aleksandra V. Zinchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Elena N. Bobrova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Miroslav I. Schetinsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Yulianna S. Govorova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

phase transitions, differential scanning calorimeter, placenta extract, autoclaving, cell suspensions, SPEV, erythrocytes, Candida albicans.

Abstract

Phase transitions in human placental extracts during cooling at -196°C and the effect of fresh and autoclaved extracts on phase transitions in cell suspensions were studied. It has been found that after cooling with the rate of 3.3(3)deg/s in cell suspensions or suspending media the process of crystallization termination developed at the stage of heating with the rate of 8.3(3)x10-3 deg/s. After dilution of the suspension of Candida albicans and SPEV cells with autoclaved extract this process was not observed. It was found that the crystallization of eutectic mixtures was developed in frozen placental extracts unlike the mixture of extracts with erythrocytes and C. albicans cells. Probl Cryobiol Cryomed 2013; 23(1):40-48.

Author Biographies

Aleksandra V. Zinchenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiophysics

Elena N. Bobrova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiophysics

Miroslav I. Schetinsky, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiophysics

Yulianna S. Govorova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiophysics

References

Virina A.M., Feygin A.M., Belousova I.I. et al. Change of composi-tion of Candida albicans cells at occurrence of resistance to polyene antibiotics. Mikrobiologiya 1976; 45 (4): 679–682.

Zinchenko A.V. The studying of phase transitions and physical states of aqueous solutions of polyatomic alcohols within the temperature range of –150°C...0°C: Abstract of the thesis of candidate of physical and mathematical sciences Kiev, 1983.

Potapov V.M. Stereochemistry Moscow: Khimiya, 1988.

Rozanov L.F., Vysekantsev I.P., Petrenko T.F. et al. Cell sensitivity of recultured cell SPEV line and Candida albicans fungi to extra- and intracellular crystallization processes. Problems of Cryobiology 2004; (3): 18–25.

Rozanova S.L., Schetinsky M.I., Rozanova E.D., Nardid O.A. Some properties and biological activity of autoclaved placental extracts. Vysokie tekhnologii, fundamentalnye i prikladnye issledovaniya 2010; 2: 90–94.

Shakhparonov M.I. Mechanisms of rapid processes in fluids Moscow: Vysshaya shkola, 1980.

Patent 5120 (Ukraine), IPC7 A61K35/50. Treatment method of human placental tissue. V.I. Grischenko, O.I. Osetskiy, G.O. Babiychuk, Yu.G. Fedchenko, M.I. Schetinsky, M.O. Osetska; Applied 07.07.2004; Publ. 15.02.2005. Bull. N2.

Kuntz I. D. Hydration of macromolecules. J Amer Chem Soc 1971; 93 (2): 514–516.

Msogoya T. J., Maerere A. P., Nzogela Y. et. al. Changes in acidity of plant growth media during heat sterilization. J Applied Biosciences 2008; 10: 488–490.

Onuaguluchi G., Ghasi S. The pharmacological basis for the use of dried placenta in traditional obstetric practice in Nigeria. J Ethnofarmacology 1996; 54 (1): 27–36.

Shibasaki T., Odagiri E., Shizume K., Ling N. Corticotropinrelea-sing factor like activity in human placental extracts. J Clin Endocrinol Metab 1982; 55: 384–386.

Shinde V., Dhalwal K., Paradkar A.R. et. al. Evaluation of in–vitro antioxidant activity of human placental extract. Pharmacology online 2006; 3: 172–179.

Togashi S., Takashi N., Kubo Y. et. al. Purification and identification of antioxidant substances in human placenta extracts. J Health Sci 2000; 46 (2): 117–125.

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Published

2013-03-15

How to Cite

Zinchenko, A. V., Bobrova, E. N., Schetinsky, M. I., & Govorova, Y. S. (2013). Influence of Autoclaved Placenta Extracts on Phase Behavior of Cell Suspensions Below 0°C. Problems of Cryobiology and Cryomedicine, 22(1), 40–48. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/8

Issue

Vol. 22 No. 1 (2012): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

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