Vitrification of Mesenchymal Stromal Cells in Alginate Microbeads

Authors

  • V. S. Zaykov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Yu. A. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. A. Trufanova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. I. Pravdyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. Yu. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

mesenchymal stromal cells, alginate microbeads, vitrification, multicomponent cryoprotective solution, step-wise exposure

Abstract

Cryopreservation of cells encapsulated in alginate microbeads is one of the important issues of tissue engineering. The present study was directed on the estimation of the effect caused by of duration of alginate encapsulated mesenchymal stromal cells (MSCs) exposure in multicomponent cryoprotective solution DEPS-1 (10% DMSO, 20% EG, 20% 1,2-PD and 0.5 M sucrose) on survival and metabolic activity after vitrification and rewarming. It was shown using of multicomponent cryoprotective solution and stepwise exposure allowed to achieve successful cryopreservation by means of vitrofication of MSCs encapsulated in alginate microspheres. The main difference between vitrification of MSCs encapsulated in microspheres vs. single cells was the longer exposure with CPA.

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Published

2024-05-21

How to Cite

Zaykov, V. S., Petrenko, Y. A., Trufanova, N. A., Pravdyuk, A. I., & Petrenko, A. Y. (2024). Vitrification of Mesenchymal Stromal Cells in Alginate Microbeads. Problems of Cryobiology and Cryomedicine, 22(1), 14–20. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/93

Issue

Vol. 22 No. 1 (2012): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 V. S. Zaykov, Yu. A. Petrenko, N. A. Trufanova, A. I. Pravdyuk, A. Yu. Petrenko

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