Permeability Coefficients of Murine Enterocyte Membranes for Water and Cryoprotectants

Authors

  • Viktoriya V. Ogurtsova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Svetlana Ye. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Igor F. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Olga I. Gordiyenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo26.03.221

Keywords:

murine enterocytes, filtration coefficient, permeability coefficient, cryoprotectants, glycerol, 1, 2-propane diol, ethylene glycol, dimethyl sulfoxide

Abstract

Osmotic response of cells and transport properties of membrane are essential for cryobiological research in terms of choosing the optimal conditions to cryopreserve the specific cell type. In the present study we have found the permeability coefficients of murine enterocytes to water and such cryoprotectants as: ethylene glycol (EG), glycerol, 1,2-propanediol (1,2-PD) and dimethyl sulfoxide (DMSO). The experimental time dependencies of cell volume revealed during their contact with hypertonic solutions of cryoprotectants were fitted with numeric solutions of nonlinear equations describing this dependence in terms of linear thermodynamics of irreversible processes. The found filtration coefficients had no significant differences in cryoprotectant solutions of 1,2-PD, DMSO and glycerol ((1.42; 1.3; 1.24)x10<sup>–14</sup> m<sup>3</sup>/N·sec, respectively) and were almost twice higher in EG solution (2.4)x10<sup>–14</sup> m<sup>3</sup>/N·sec). The membranes of murine enterocytes showed the highest penetration rate for EG 4.79x10<sup>–7</sup> m/s which was probably due to its negative effect on enterocyte membranes. Permeability coefficient for 1,2-PD, DMSO and glycerol was (0.672; 0.530; 0.134)x10<sup>–7</sup> m/s, respectively. The obtained data can be used in selection of the most proper regimen of cryopreservation for these cells.

 

Probl Cryobiol Cryomed 2016; 26(3): 221–228

Author Biographies

Viktoriya V. Ogurtsova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Low Temperature Preservation

Svetlana Ye. Kovalenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Low Temperature Preservation

Igor F. Kovalenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Low Temperature Preservation

Olga I. Gordiyenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Low Temperature Preservation

References

Carter J.H., Carter H., Nussbaum J., Eichholz A. Isolation of hamster intestinal epithelial cells using hypoosmotic media and PVP. J Cell Physiol 1982; 111 (1): 55–67. CrossRef PubMed

Dumont F., Marechal P.A., Gervais P. Influence of cooling rate on Saccharomyces cerevisiae destruction during freezing: unexpected viability at ultrarapid cooling rates. Cryobiology 2003; 46: 33–42. CrossRef

Gordienko O.I., Gordienko E.O., Linnik T.P., Kompaniets A.M. Mechanisms of cryoprotectant penetration through erythro-cyte membranes. Problems of Cryobiology 2002; 4: 9–15.

Gordienko O.I., Linnik T.P., Gordienko E.O. Erythrocyte membrane permeability for a series of diols. Bioelectrochemistry 2004; 62(2): 115–118. CrossRef PubMed

Gordiyenko Ye.O., Pushkar N.S. Physical basis for low temperature preservation of cell suspensions. Kyiv: Naukova dumka; 1994.

Mazur P. Theoretical and experimental effects of cooling and warming velocity on the survival of frozen and thawed cells. Cryobiology 1966; 2: 181–192. CrossRef

Meyrial V., Laize V., Gobin R. et al. Existence of a tightly regulated water channel in Saccharomyces cerevisiae. Eur J Biochem 2001; 268: 334–343. CrossRef PubMed

Morris G.J., Coulson G.E., Clarke K.-J. Freezing injury in Saccharomyces cerevisiae: the effect of growth conditions. Cryobiology 1988; 25: 471–482. CrossRef

Muldrew K., McGann L.E. Mechanisms of intracellular ice formation. Biophys J 1990; 57: 525–532. CrossRef

Ogurtsova V.V., Kovalenko S.Ye., Kovalenko I.F., Gordiyenko O.I. Determination of osmotically inactive volume of murine enterocytes. Probl Cryobiol Cryomed 2016; 26(1): 93–97. CrossRef

Sakun O.V., Kovalenko I.F., Sirenko A.Yu. et al. Membrane permeability coefficients of yeast Saccharomyces cerevisiae to water and cryoprotectants. V.N. Karazin KhNU Bull Series Biol 2008; 7(814): 140–146.

Tanghe A., Van Dijck P., Colavizza D., Thevelein J. M. Aquaporin-mediated improvement of freeze tolerance of Saccharomyces cerevisiae is restricted to rapid freezing conditions. Appl Environ Microbiol 2004; 70 (6): 3377–3382. CrossRef PubMed

Downloads

Published

2016-09-23

How to Cite

Ogurtsova, V. V., Kovalenko, S. Y., Kovalenko, I. F., & Gordiyenko, O. I. (2016). Permeability Coefficients of Murine Enterocyte Membranes for Water and Cryoprotectants. Problems of Cryobiology and Cryomedicine, 26(3), 221–228. https://doi.org/10.15407/cryo26.03.221

Issue

Section

Theoretical and Experimental Cryobiology