Effect of Respiration and Vascular Tone on Heart Rate Variability in Rats


  • Nikolay A. Chizh Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv




heart rate variability, spectral analysis, respiration, vascular tone, rats


Basing on findings of a combined study (electrocardiography, pneumotachography, direct sphygmography) the paper discusses the effect of respiration and vascular tone on heart rate variability (HRV) in rats and presents a physiological interpretation of the selected spectral ranges. Studying the HRV in conscious rats showed the lower HF-wave range limit to be not higher than 0.8 Hz, and the upper one not less than 2.5 Hz;  under stress this index increased up to 3 Hz. The experiments showed that conscious rats were characterized with the ‘package-like’ respiration (from 2 to 15–20 s). The respiratory rate in each ‘package’ was different, therefore two or more peaks might be recorded at high-frequency spectral range. This fact confirmed the existence of several oscillators-pacemakers of respiratory center. In the anaesthetised rats the vasomotor waves were established to affect the variability of respiratory and heart rates within the range of 0.25–0.5 Hz with 0.4 Hz peak. Therefore the lower limit of the average MF waves should not exceed 0.25 Hz.


Probl Cryobiol Cryomed 2016; 26(3): 260–270

Author Biography

Nikolay A. Chizh, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Experimental Cryomedicine


Baevsky R.M., Ivanov G.G., Chireykin L.V. et al. Analysis of heart rate variability using different electrocardiographic systems. Vestnik Aritmologii 2001; (24): 65–87.

Bartsch R., Liu K., Ma Q., Ivanov P. Three independent forms of cardio-respiratory coupling: transitions across sleep stages. Computing in Cardiology 2014; 41: 781–784.

Chizh N.A. Parameters of spectral analysis of heart rate variability in rats. Probl Cryobiol Cryomed 2015; 25 (3): 235–245.

Chizh N.A. Ways of registering electrocardiograms in rats for analyzing heart rate variability. Exp Clin Med 2015; 68 (3): 44–47.

Janssen B.J., Oosting J., Slaaf D.W. et al. Hemodynamic basis of oscillations in systemic arterial pressure in conscious rats. Amer J Physiol 1995; 269: 62–71.

Julien C. The enigma of Mayer waves: facts and models. Cardiovasc Res 2006; 70(1): 12–21. CrossRef PubMed

Khayutin V.M., Lukoshkova E.V. Heart rate oscillation: spectral analysis. Vestnik Aritmologii 2002; (26): 10–18.

Kiselev A.R. Oscillatory processes in vegetative regulation of cardiovascular system (review). Saratov Journal of Medical Scientific Research 2011; 7(1): 34–39.

Korkushko O.V., Pisaruk A.V., Shatilo V.B. et al. Analysis of heart rate variability in clinical practice (age-sensitive). Kiev; 2002.

Korolev I.B., Kotelnikov V.N., Osipov I.O. Comparative assessment of state of sympathetic and parasympathetic parts of autonomic nervous system at high blood pressure level in rats. Science and World 2014; 7(3): 122–127.

Kositskii G.I. Respiratory waves of blood pressure in human subjects. Bull Exp Biol Med 1958; 45: 154–157. CrossRef

Kotelnikov S.A., Nozdrachev A.D., Odinak M.M. et al. Ideas about mechanisms of heart rate variability. Human Physiology 2002; 28(1): 130–143.

Kumae T. Assessment of training effects on autonomic modulation of the cardiovascular system in mature rats using power spectral analysis of heart rate variability. Environ Health Prev Med 2012; 17: 415–422. CrossRef PubMed

Lutsenko D.G., Shylo A.V., Marchenko L.N. et al. Peculiarities of heart rhythm regulation at different types of cold acclimation in rats. Probl Cryobiol Cryomed 2013; 23(2): 105–115.

Nilsson H, Aalkjaer C. Vasomotion: mechanisms and physiological importance. Mol Interv 2003; 3(2): 79–89. CrossRef PubMed

Ramaekers D., Beckers F., Demeulemeester H., Aubert A. Cardiovascular autonomic function in conscious rats: a novel approach to facilitate stationary conditions. Ann Noninvasive Electrocardiol 2002; 4: 307–318. CrossRef

Standards of heart rate variability. European Heart Journal 1996; 17: 354–381. CrossRef

Takakura A.C., Moreira T.S., Colombari E., Peripheral chemoreceptor inputs to retrotrapezoid nucleus (RTN) CO2-sensitive neurons in rats. J Physiol 2006; 572: 503–523. CrossRef PubMed

Yabluchansky N.I., Martynenko A.V. Heart rate variability to help practising physician. Kharkov; 2010.




How to Cite

Chizh, N. A. (2016). Effect of Respiration and Vascular Tone on Heart Rate Variability in Rats. Problems of Cryobiology and Cryomedicine, 26(3), 260–270. https://doi.org/10.15407/cryo26.03.260



Cryomedicine, Clinical and Experimental Transplantology