@article{Bohuslavskyi_Alabedalkarim_Pakhomov_Bozhok_2016, title={Effect of Incubation of PK-15 Line Cells with Cryoprotectants on Amount of Gal-alpha-1,3-Gal Epitopes}, volume={26}, url={http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1242}, DOI={10.15407/cryo26.04.331}, abstractNote={<p>Transplantation of bioprostheses derived from bovine and porcine tissues is an advanced method of correcting defects in blood vessels and valves, however when using it a hyperacute immune response associated with the presence of Gal-alpha- 1,3-Gal (α-Gal epitope) on the surface of cell membranes can develop. We have studied the effect of penetrating and nonpenetrating cryoprotectants on the amount of α-Gal epitopes on membranes of PK-15 cell line of porcine origin. Cell labeling with BSIB4 lectin conjugated with FITC and flow cytometry revealed a significant increase in the number of fluorescent cells after incubation with various concentrations of dimethyl sulfoxide and polyethylene glycol with MW of 1500 (PEG-1500). The revealed effect may be associated with an additional exposure of α-Gal epitopes on cell membrane, locally deformed due to the action of cryoprotectants.</p><p><em>Probl Cryobiol Cryomed 2016; 26(4): 331–339</em><em></em></p>}, number={4}, journal={Problems of Cryobiology and Cryomedicine}, author={Bohuslavskyi, Konstantin I. and Alabedalkarim, Natalia M. and Pakhomov, Alexsandr V. and Bozhok, Galyna A.}, year={2016}, month={Dec.}, pages={331–339} }