@article{Gurina_Kirilyuk_2012, title={Temperature Ranges of Phase Transformations in the Cryoprotective Media Components Determined by Thermoplastic Deformation Method}, volume={22}, url={http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/46}, abstractNote={<p>Phase transformations in multicomponent cryoprotective media and their components were investigated during freeze-thawing using the method of thermoplastic deformation. The research objects included solution of cryoprotectant DMSO (10%) and the components of cryoprotective media being often utilized as a solvents for biologicals’ cryoprotective media (distilled water, physiological saline and the culture media: medium 199, Hanks’ solution, DMEM). The paper represents the temperature ranges of crystallization (melting) of eutectic concentration mixture for DMSO solution (from –87 to –67°C), eutectic crystallization (melting) of physiological solution and the culture media (from –37 to –21°C), recrystallization before melting of bulk ice (from –21 to –15°C) and following melting of bulk ice (from –15 to –4.5°C).</p>}, number={4}, journal={Problems of Cryobiology and Cryomedicine}, author={Gurina, T. M. and Kirilyuk, A. L.}, year={2012}, month={Dec.}, pages={410–422} }