@article{Nardid_2014, title={Effect of Low Temperatures on Protein Systems}, volume={24}, url={http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/555}, DOI={10.15407/cryo24.02.083}, abstractNote={<p>The review concerns the main issues of preserving the structure and function of proteins either isolated or as a part of biological tissues during hypothermic and cryogenic storage. The role of biomacromolecules’ dehydration, as a consequence of water freeze-out in proteins cryodamage during freeze-thawing is discussed, as well as peculiarities of protein low-temperature denaturation, associated with entropic nature of hydrophobic interactions. Low temperature injuries in biomacromolecules, their<br />reversibility with considering the role of water in structure instability of cooled or frozen biopolymers are described. Potential ways to protect biomacromolecules during freezing are considered.</p><p><em><br /></em></p><p><em>Probl Cryobiol Cryomed 2014; 24(2):83-101.</em></p>}, number={2}, journal={Problems of Cryobiology and Cryomedicine}, author={Nardid, Oleg A.}, year={2014}, month={Jun.}, pages={83–101} }