@article{Kudokotseva_Volina_Lomakin_2016, title={Morphological Features of Spleen Tissue After Introduction of Fluorouracil and Cryopreserved Cord Blood Nucleated Cells}, volume={26}, url={http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/942}, DOI={10.15407/cryo26.03.249}, abstractNote={<p>We studied the effect of administered cryopreserved nucleated cells of human cord blood (cCBNCs) on murine spleen tissue morphology under hemo- and immunosuppression induced by a single intraperitoneal injection of 5-fluorouracil (5-FU) antimetabolite of nucleic metabolism in the maximum tolerated dose. Progressing changes, typical for a damage caused by toxic substances, such as: congestion, exudation and leukocytes infiltration of spleen pulp; accumulation of macrophages either with phagocytized lymphocytes or their fragments; degenerative and necrotic changes in tissue elements of the pulp and follicles were revealed in spleen following  5‑FU introduction within entire observation period (16 days). A high degree of murine spleen reactivity in response to the administration of cryopreserved cord blood cells was found. To day 2 after cCBNCs administration we observed structural rearrangements in spleen, and already 10 days later 5-FU antimetabolite toxic injury the organ structure was back to the norm.</p><p> </p><p><em>Probl Cryobiol Cryomed 2016; 26(3):</em><em> 249–258</em><em></em></p>}, number={3}, journal={Problems of Cryobiology and Cryomedicine}, author={Kudokotseva, Olga V. and Volina, Viktoriya V. and Lomakin, Ivan I.}, year={2016}, month={Sep.}, pages={249–259} }