TY - JOUR AU - Chernobai, N. A. AU - Pakhomov, A. V. AU - Kovalenko, I. F. AU - Bozhok, G. A. AU - Kovalenko, S. Ye. AU - Rozanov, L. F. PY - 2010/06/07 Y2 - 2024/03/30 TI - Temperature Dependence of Testes Intersticium Cell Membrane Permeability for Cryoprotectant Molecules JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 20 IS - 2 SE - Theoretical and Experimental Cryobiology DO - UR - http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/204 SP - 153-158 AB - <p>Osmotic behavior of the rat testis intersticium cells in cryoprotectants solutions – substances belonging to alcohols (ethylene glycol (EG), glycerol and 1,2-butane diol (1,2-BD)), oxides (dimethyl sulfoxide (Me<sub>2</sub>SO)) and amides (dimethyl formamide (DMFA)) was studied. Permeability coefficients of testis cells for these cryoprotectants were determined at 35, 20 and 5°C by volumetric analysis and the Kedem-Kachalsky modified physico-mathematical model. The calculated values of the activation energies for EG, glycerol, 1,2-BD, Me<sub>2</sub>SO and DMFA molecules are 53.28, 46.92, 36.33, 58.89, 62.99 kJ/mol, respectively. Testis cell membranes were established to be equally well permeable for EG, glycerol, 1,2-BD and Me<sub>2</sub>SO, and their permeability for DMFA at 35°C was comparable with that for water molecules.</p> ER -