TY - JOUR AU - Malyukina, Margarita Yu. AU - Kavok, Nataliya S. AU - Borovoy, Igor A. AU - Averchenko, Katerina A. PY - 2012/06/20 Y2 - 2024/03/29 TI - Sensitivity of Hepatocyte Mitochondria Transmembrane Potential to Regulation with Phenylephrine after Exposure in Dimethyl Sulfoxide and 1,2-Propanediol Solutions, and Freeze-Thawing JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 23 IS - 2 SE - Theoretical and Experimental Cryobiology DO - UR - http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/31 SP - 143-151 AB - <p>Intensity of JC-1 probe aggregates fluorescence and agonist-induced changes in mitochondria transmembrane potential of rat hepatocytes prior to and after freeze-thawing were assessed by single-cell microfluorimetry. It was shown that incubation of the cells in 10% dimethyl sulfoxide (DMSO) and 1,2-propanediol (1,2-PD) solutions within an hour and the following washing decreased the fluorescence of dye aggregates. After freeze-thawing of cells with the cryoprotectants (2-step freezing down to the temperature of liquid nitrogen, thawing and washing-out the cryoprotectants) the intensity of dye aggregates fluorescence decreased down to 83 ± 6% of control in case of DMSO, and in the case of 1,2-PD the index made less than 10%. Unlike the fresh cell suspension where the rise of aggregates fluorescence intensity by 30% was found in response to short-term stimulation with phenylephrine (10<sup>-5</sup> M) the hepatocyte suspension incubated in DMSO solution showed no response. After freeze-thawing of cells we revealed a partial recovery of their sensitivity to the hormone effect, but the response was recorded only after increasing the hormone concentration up to 10<sup>-4</sup> M.<i>Probl Cryobiol Cryomed 2013; 23(2):143-151.</i></p> ER -