TY - JOUR AU - Zinchenko, Vasiliy D. AU - Goryachaya, Irina P. AU - Buryak, Irina A. AU - Vysekantsev, Igor P. PY - 2014/03/25 Y2 - 2024/03/28 TI - Pretreatment with Ozone Elevates the Resistance of Saccharomyces cerevisiae Yeast Membranes during Freeze-Thawing JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 24 IS - 1 SE - Theoretical and Experimental Cryobiology DO - 10.15407/cryo24.01.038 UR - http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/545 SP - 38-46 AB - <p>Тhe effect of ozone on injury of <em>Saccharomyces cerevisiae</em> yeast membranes following freezing (down to –196°C) and warming (up to 30°C) was investigated using fluorescent staining and flow cytometry. It has been shown that freeze-thawing of <em>S. cerevisiae</em> cells in physiological solution resulted in the injury of membranes in 65.6% of total cells population. Treatment of the cells with ozone in a dose of 29–240 рmol О<sub>3</sub>/10<sup>6</sup> cells prior to freezing allowed to reduce significantly the number of damaged cells: (16.1 ± 0.9)% of total population. The observed effect was explained by cross-adaptation caused by ozone introduction: the adaptive response to slight oxidative stress initiated the genetically determined mechanisms of responces to other stresses, including the one caused by freeze-thawing.</p><p><em><br /></em></p><p><em>Probl Cryobiol Cryomed 2014; 24(1):38–46</em>.</p> ER -