TY - JOUR AU - Mikson, Konstantin B. AU - Revenko, Elena B. AU - Gapon, Anna A. PY - 2014/06/20 Y2 - 2024/03/29 TI - Effect of Cryoprotective Solutions on Survival and Teratogenicity of Prussian Carp Embryos (Carassius auratus gibelio Bloch, 1783) JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 24 IS - 2 SE - Theoretical and Experimental Cryobiology DO - 10.15407/cryo24.02.149 UR - http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/559 SP - 149-156 AB - <p>There was studied the effect of cryoprotective solutions, belonging to different classes of chemicals, on the survival<br />and abnormal development of Prussian carp (Carassius auratus gibelio Bloch, 1783) embryos at the stage of development corresponding to 6 somites. The embryos were incubated during 60 minutes in 10% solutions of cryoprotectants dimethyl sulfoxide (DMSO), 1,2-propane diol (1,2-PD), 1,3-propane diol (1,3 PD), 1,3-butane diol (1,3-DB), 1,4-butane diol (1,4-BD), 1,2-metaoxyethane (1,2-MOE), glycerol, ethylene glycol (EG), methanol, polyethyleneoxide (PEO-1500) and sucrose. Thereafter, the embryos were washed and traced to the stage of development of free-swimming larvae. The highest survival rates for the embryos of Prussian carp (54–67%) was observed in 10% solutions of 1,2-PD, 1,3-PD, EG, PEO-1500, and sucrose, providing the preservation of normally developing embryos close to the control level. Cryoprotective solutions of 1,3-BD, 1,4-BD had higher toxicity: the survival of embryos in those made 40–49%. In the solutions of 1,2-MOE, DMSO, glycerol and methanol cryoprotective agents the survival rate of embryos was minimal. The highest level of teratogenic embryos was observed after incubation in the cryoprotectant solutions of 1,2-MOE and methanol.</p><p><em><br /></em></p><p><em>Probl Cryobiol Cryomed 2014; 24(2):149-156.</em></p> ER -