TY - JOUR AU - Pakhomova, Yuliya S. AU - Chekanova, Valentina V. AU - Kompaniets, Antonina M. PY - 2013/03/15 Y2 - 2024/03/29 TI - Cryoprotective Properties of Solutions Based on Non-Penetrative OEGn = 25 Combined with Penetrating Cryoprotectants During Freezing of Human Erythrocytes JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 23 IS - 1 SE - Theoretical and Experimental Cryobiology DO - UR - http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/7 SP - 26-39 AB - <p>Cryoprotective efficiency of сryopreservatives, based on combination of extracelular cryoprotectant oxyethylated glycerol with polymerization degree n = 25 (OEG<sub>n=25</sub>) with penetrating cryoprotectants 1,2-propanediol (1,2-PD), dimethyl sulphoxide (DMSO) and dimethyl acetamide (DMAc) during red blood cells freezing was studied. Freeze-thawing in solutions of OEG<sub>n=25</sub> and 1,2-PD, DMSO and DMAc mixed in 5:1 ratio, as well as with DMAc in ratios of 2:1 and 1:1 resulted in the reduction of osmotic fragility of red blood cells comparing to non-treated cells. The highest level of post-thaw survival of red blood cells according to indices of hemolysis and content of free hemoglobin was found for 30% OEG<sub>n=25</sub> solution.<i>Probl Cryobiol Cryomed 2013; 23(1):26-39.</i></p> ER -