TY - JOUR AU - Gulevsky, Aleksandr K. AU - Grischenkova, Elena O. AU - Relina, Liana I. AU - Tretyak, Diana V. PY - 2015/12/21 Y2 - 2024/03/29 TI - PEG-6000 Induced Precipitation of Proteins from Cold-Acclimated Tenebrio molitor larvae JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 25 IS - 4 SE - Theoretical and Experimental Cryobiology DO - 10.15407/cryo25.04.350 UR - http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/801 SP - 350-358 AB - <p>Spectra of PEG-6000-precipitated proteins from cold-acclimated and non-acclimated Tenebrio molitor larvae were comparatively studied. The composition of precipitated proteins from cold-acclimated and non-acclimated T. molitor larvae was shown as differed qualitatively. In particular, the protein spectra of filtrates from cold-acclimated larvae had proteins with molecular weight of 11 kDa, which were absent in non-acclimated insects. There was observed a significant difference in precipitate content at polyethyleneglycol concentrations of 12% and higher during protein precipitation from homogenates of cold-acclimated and non-acclimated larvae. Proteins from the filtrates of cold-acclimated larvae in the presence of 6% PEG-6000 were precipitated by 44 and 56% greater, than those from non-acclimated species at 22 and 4°C, respectively. The increasing of PEG-6000 concentration from 4 to 6% resulted in appearance in the precipitates from cold-acclimated larvae of proteins with molecular-weight range of 30–42.5 kDa, which were absent in non-acclimated insects.</p><p><em><br /></em></p><p><em>Probl Cryobiol Cryomed 2015; 25(4):350-358.</em></p> ER -