http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/issue/feedProblems of Cryobiology and Cryomedicine2024-05-19T14:41:04+02:00Anatoliy M. Goltsevjournal@cryo.org.uaOpen Journal Systems<p>The Journal was established in 1985. Till 1991 it was published under title Kriobiologia, and Problemy kriobiologii/Problems of Cryobiology from 1991 till 2012. (<a href="/journal/index.php/probl-cryobiol-cryomed/at_a_glance">Journal at a Glance</a>)</p> <p>The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine.</p> <p>The Editorial Board takes the responsibility to provide a comprehensive peer-review of all submitted manuscripts. The Journal team strictly follows publishing ethics and standards to ensure high quality of scholarly publications. The Journal endorses the International Committee of Medical Journal Editors (ICMJE) <a href="http://www.icmje.org/recommendations/" target="_self">Recommendations for the Conduct, Reporting, Editing and Publication of Scholarly Work in Medical Journals</a>. (<a href="/journal/index.php/probl-cryobiol-cryomed/pubethics">Read more about Publishing Policy and Ethics</a>).</p> <p>Members of our Editorial Board are the specialists in various fields of low temperature biology and medicine from Ukraine and all over the world. (<a href="https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/about/editorialTeam">Editorial Board</a>).</p> <p>We do not charge the authors for article submission and/or processing and do not provide off-prints. The operation of the Journal is supported by National Academy of Sciences of Ukraine.</p> <p>Articles published in Problems of Cryobiology and Cryomedicine are Open-Access articles distributed under the terms and conditions of the Creative Commons Attribution License (<a href="http://creativecommons.org/licenses/by/4.0/">CC BY</a>). We provide immediate open access to its content adhering to the principle that making research freely available to the public supports a greater global exchange of knowledge, increase visibility and readership. Use and distribution of the publications are permitted, retaining the credits to the authors and the Journal. The Journal holds the exclusive license to be the first publisher of the articles in print form and on-going non-exclusive license to disseminate the published content in various ways. The published version of the article could be deposited by authors to institutional or public repositories immediately on publication, stating a link to the URL of the published article on the journal's web-site.</p> <p>Our Journal is indexed/abstracted in <a href="http://www.scopus.com">Scopus</a>, <a href="http://thomsonreuters.com/en/products-services/scholarly-scientific-research/scholarly-search-and-discovery/zoological-record.html">Zoological Records</a>, <a href="http://www.cas.org/">Chemical Abstracts</a>, <a href="https://www.ebscohost.com/discovery?_ga=2.208532498.1058102411.1521647075-1463563202.1521647075">EBSCOhost</a>, <a href="http://bd.viniti.ru/">VINITI</a>, <a href="http://www.irbis-nbuv.gov.ua/cgi-bin/irbis64r_81/cgiirbis_64.exe?C21COM=F&I21DBN=REF_EX&P21DBN=REF&S21CNR=20&Z21ID=">Dzherelo </a>(Ukrainian Scientific Abstracts) and deposited in <a href="http://www.irbis-nbuv.gov.ua/cgi-bin/irbis_nbuv/cgiirbis_64.exe?Z21ID=&I21DBN=UJRN&P21DBN=UJRN&S21STN=1&S21REF=10&S21FMT=juu_all&C21COM=S&S21CNR=20&S21P01=0&S21P02=0&S21P03=PREF=&S21COLORTERMS=0&S21STR=KrioBiol">Vernadsky National Library of Ukraine Repository</a> (2002-now) and Google Books (<a href="https://books.google.co.jp/books?id=CSfuSZsuprkC&dq=kriobiologiya&hl=uk&source=gbs_all_issues_r&cad=1&atm_aiy=1970#all_issues_anchor">1975-1990</a>; <a href="https://books.google.com.ua/books?id=B326AAAAIAAJ&dq=problems+of+cryobiology&hl=uk&source=gbs_navlinks_s">1990-2002</a>).</p> <div id="gtx-anchor" style="position: absolute; visibility: hidden; left: 1473.54px; top: 296.181px; width: 9.88892px; height: 18.8889px;"> </div> <div class="jfk-bubble gtx-bubble" style="visibility: visible; left: 575px; top: 325px; opacity: 1;" role="alertdialog" aria-describedby="bubble-2"> <div id="bubble-2" class="jfk-bubble-content-id"> <div id="gtx-host" style="min-width: 200px; max-width: 400px;"> </div> </div> <div class="jfk-bubble-closebtn-id jfk-bubble-closebtn" tabindex="0" role="button" aria-label="Close"> </div> <div class="jfk-bubble-arrow-id jfk-bubble-arrow jfk-bubble-arrowup" style="left: 1470.56px;"> <div class="jfk-bubble-arrowimplbefore"> </div> <div class="jfk-bubble-arrowimplafter"> </div> </div> </div>http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1956Cover of the Issue2024-05-19T09:46:00+02:00Editorial Board Problems of Cryobiology and Cryomedicinejournal@cryo.org.ua2024-05-19T00:00:00+02:00Copyright (c) 2024 http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1957Editorial Information2024-05-19T10:10:31+02:00Editorial Board Problems of Cryobiology and Cryomedicinejournal@cryo.org.ua2024-05-19T00:00:00+02:00Copyright (c) 2024 http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1958Table of Contents2024-05-19T10:13:07+02:00Editorial Board Problems of Cryobiology and Cryomedicinejournal@cryo.org.ua<p><em>Probl Cryobiol Cryomed 2024; 34(</em><em>1</em><em>):2</em></p>2024-05-19T00:00:00+02:00Copyright (c) 2024 http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1959Thermal Field Monitoring When Exposing Soft Tissues to Low Temperatures: Thermography Prospects and Limitations2024-05-19T10:20:32+02:00Gennadiy Kovalovg.o.kovalyov@nas.gov.uaMykola Chyzhprobl.cryobiol.cryomed@gmail.comVyacheslav Globaprobl.cryobiol.cryomed@gmail.comGalyna Shustakovaprobl.cryobiol.cryomed@gmail.comYuliya Fomenkoprobl.cryobiol.cryomed@gmail.comEduard Gordiyenkoprobl.cryobiol.cryomed@gmail.com<p>The review analyzes the existing tools for monitoring the dynamics of thermal fi elds when exposing the soft tissues to low temperatures. Features of contact and non-contact temperature measurements have been considered, their capabilities and limitations have been noted. There was substantiated the need to develop the procedures of intra-operative temperature control. Special attention has been paid to the non-contact non-invasive infrared thermography. This method has been shown to be applied for intra-operative monitoring of the movement of the ice lump edge on the surface of tissues, detection of a disordered thermal symmetry of the ice spot, thermal fi eld dynamics on the surface of tissues inside and outside the area of the operative zone. However, thermal imaging control of the dynamics of the primary necrosis zone and the ice ball edge in the volume of tissues is possible only under certain parameters of cryoimpact, for example, with a short-term cooling of tissues with a quasi-point nitrogen cryoapplicator. The possibility of using thermography at other stages of cryosurgery is also considered, i. e. as the method of additional diagnosis at the stage of surgery planning, as well as during the post-surgery period to control healing, scarring, etc.</p> <p> </p> <p><em>Probl Cryobiol Cryomed 2024; 34(1):003–018</em></p>2024-05-19T00:00:00+02:00Copyright (c) 2024 http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1960Amphiphilic Compounds as Antihemolytic Agents: Problems and Prospects2024-05-19T10:48:04+02:00Olena Chabanenkochabanenkoolena@gmail.comNatalia Yershovaprobl.cryobiol.cryomed@gmail.comNataliia Orlovaprobl.cryobiol.cryomed@gmail.comOlena Laptiyprobl.cryobiol.cryomed@gmail.comNataliia Shpakovaprobl.cryobiol.cryomed@gmail.com<p>In this research the effect of surface-active substances belonging to different classes of amphiphilic compounds (cationic trifluoperazine (TFP), anionic sodium decyl sulfate (C10) and nonionic decyl-β,D-glucopyranoside (DGP)) on the level of damage of human erythrocytes during posthypertonic shock (PHS) and glycerol removal from cells frozen to –196°C was study. All amphiphilic compounds in low concentrations (at which the antihemolytic activity is approximately 45% under the conditions of PHS cells) show the same efficiency when removing glycerol from thawed cells. It was established that among amphiphilic compounds in effective concentrations the maximum antihemolytic (AHmax) activity was shown by DGP (74%) during the removal of glycerol from thawed cells and C10 (74%) under conditions of PHS of erythrocytes, while the AHmax activity indicators of TFP were comparable in both cases. A feature of the effectiveness of C10 under the conditions of deglycerolization of cryopreserved erythrocytes is almost the same AH when used in both concentrations. The method of flow cytometry showed that the amount of annexin-labeled cells depended on the concentration of C10 both in physiological solution and under PHS conditions.</p> <p> </p> <p><em>Probl Cryobiol Cryomed 2024; 34(1):019–032</em></p>2024-05-19T00:00:00+02:00Copyright (c) 2024 http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1961Cryopreserved Umbilical Cord Extract Restores the Skin Structure in Ovariectomized Rats2024-05-19T11:16:37+02:00Oksana Falkoprobl.cryobiol.cryomed@gmail.comNadiia Shevchenkoshevchenko_nadyusha@ukr.netViktoria Volinaprobl.cryobiol.cryomed@gmail.comOlena Terekhovaprobl.cryobiol.cryomed@gmail.comOlga Prokopyukprobl.cryobiol.cryomed@gmail.comAnna Kaverinskaprobl.cryobiol.cryomed@gmail.comVolodymyr Karpenkoprobl.cryobiol.cryomed@gmail.comVolodymyr Prokopyukprobl.cryobiol.cryomed@gmail.com<p>The research covers the specific effect of human umbilical cord cryoextract and the culture medium of its mesenchymal stromal cells in comparison with synthetic hyaluronic acid on the skin regeneration in female rats with simulated aging. The specified substances were administered intradermally to ovariectomized animals. The injection of human umbilical cord cryoextract into animals with a model of skin aging was established to restore the skin structure already 2 weeks after the injections. Thickening of the epidermis and dermis, phenomenon of cell proliferation, and an increased number of collagen fibers as well as blood vessels were observed. Four weeks after the last injection, the skin condition was normalized to the level of intact animals. Administration of the culture medium of mesenchymal stromal cells of the umbilical cord also led to an increased number of collagen fibers, cell proliferation, contouring of skin layers, but had a less pronounced effect compared to the injections of cryoextract on the indices of the dermis and epidermis thickness. Injections of synthetic hyaluronic acid led to "rejuvenation" of the upper layers of skin and had a less pronounced manifestation compared to the effect of human umbilical cord derivatives.</p> <p> </p> <p><em>Probl Cryobiol Cryomed 2024; 34(1):033–044</em></p>2024-05-19T00:00:00+02:00Copyright (c) 2024 http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1962Intra-Operative Infrared Thermography of Ocular Surface During Cryodestruction of Epibulbar Tumours2024-05-19T11:58:57+02:00Oleg Zadorozhnyyzadoroleg2@gmail.comOleksandr Buikoprobl.cryobiol.cryomed@gmail.comNataliya Pasyechnikovaprobl.cryobiol.cryomed@gmail.com<p>The paper presents the possibilities of intraoperative use of infrared (IR) thermography to image the temperature distribution of the outer ocular surface during cryodestruction of conjunctival tumours with localization in the ciliary body projection to determine an individual freezing regimen. IR thermography was carried out using a portable device for infrared real-time thermography. Thermal fields were visualized, and the duration of cooling and warming of the structures of the outer ocular surface around the area of freezing, including the cornea, were recorded. It was found that intraoperative IR thermography during cryodestruction of epibulbar tumours in the projection of the ciliary body allows real-time noninvasive imaging of the temperature fi eld dynamics around the freezing zone, which can be used to select individual cooling exposure to reduce the risk of complications by avoiding excessive cooling of intraocular structures. It was also determined that IR thermography makes it possible to assess the individual dynamics of rewarming of the ocular tissues to the initial level if a repeated cycle of tumour cryodestruction is necessary.</p> <p> </p> <p><em>Probl Cryobiol Cryomed 2024; 34(1):045–053</em></p>2024-05-19T00:00:00+02:00Copyright (c) 2024 http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1963Dependence of Ascorbic Acid Content in Nettle Leaf Extracts on Cryoextraction Conditions2024-05-19T13:33:34+02:00Ivan Shcheniavskyiivanhou11@gmail.comYuliia Akhatovaprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(1):054–058</em></p>2024-05-19T00:00:00+02:00Copyright (c) 2024 http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1964Information about the All-Ukrainian Scientific and Practical Conference ‘Stress-Induced Immune Disorders and Their Consequences in Wartime’2024-05-19T13:50:42+02:00Editorial Board Problems of Cryobiology and Cryomedicinejournal@cryo.org.ua<p><em>Probl Cryobiol Cryomed 2024; 34(1):59-59</em></p>2024-05-19T00:00:00+02:00Copyright (c) 2024 http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/196570th birthday of Oleksandr Petrenko2024-05-19T14:24:51+02:00Editorial Board Problems of Cryobiology and Cryomedicinejournal@cryo.org.ua<p><em>Probl Cryobiol Cryomed 2024; 34(1):60-62</em></p>2024-05-19T00:00:00+02:00Copyright (c) 2024 http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/196675th birthday of Tetiana Bondarenko2024-05-19T14:28:08+02:00Editorial Board Problems of Cryobiology and Cryomedicinejournal@cryo.org.ua<p><em>Probl Cryobiol Cryomed 2024; 34(1):63-64</em></p>2024-05-19T00:00:00+02:00Copyright (c) 2024 http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1967Instruction for Authors2024-05-19T14:31:11+02:00Editorial Board Problems of Cryobiology and Cryomedicinejournal@cryo.org.ua<p><em>Probl Cryobiol Cryomed 2024; 34(1):67-68</em></p>2024-05-19T00:00:00+02:00Copyright (c) 2024 http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1968Backmatter2024-05-19T14:34:33+02:00Editorial Board Problems of Cryobiology and Cryomedicinejournal@cryo.org.ua2024-05-19T00:00:00+02:00Copyright (c) 2024