Cryopreservation of Newborn Mice Adrenal Cell Suspension: 1. Effect of DMSO Concentration as a Component of Cryoprotective Medium
Keywords:cryopreservation, adrenal glands, newborn mice, DMSO
Influence of DMSO concentrations as a component of cryopreservative medium on viability, survival and functional activity of the newborn mice adrenal cells after freeze-thawing cycle has been established in this study. Maximal values of viability (63.7; 66.7%) and survival (78.3; 95.7%) are observed after freezing of newborn mice adrenal cell suspension (ACS) in the medium with 15 and 20% DMSO. Regardless the high values of ACS viability and survival after freeze-thawing cycle, none of the DMSO concentrations being used was not capable to preserve proper post-thaw cell proliferation and hormone production.
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Copyright (c) 2020 I. V. Tamarina, G. A. Bozhok, T. M. Gurina, N. F. Gubina, T. P. Bondarenko
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