Role of Forming Aggregates in Post-Thaw Survival of Isolated Neural Cells from Newborn Rats

Authors

  • A. N. Sukach Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov G.S. Skovoroda Kharkov National Pedagogical University, Kharkov
  • T. D. Lyashenko G.S. Skovoroda Kharkov National Pedagogical University, Kharkov

Keywords:

neural cells of newborn rats, multicellular aggregates, survival, viability, cryopreservation, cultivation

Abstract

The role of formation of newborn rat neural cell aggregates on cell recovery after isolation and cryopreservation was studied. Short-term cultivation of neural cells within aggregates eliminate the damages that appear during cell isolation and possibly protects from apoptosis. It was shown that freezing of neural cells within aggregates under protection of 10% DMSO is effective for storage of stem/progenitor and differentiated neural cells. Maintenance of integrity of neural cell aggregates after cryopreservation is a necessary condition for survival in culture.

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Published

2011-12-19

How to Cite

Sukach, A. N., & Lyashenko, T. D. (2011). Role of Forming Aggregates in Post-Thaw Survival of Isolated Neural Cells from Newborn Rats. Problems of Cryobiology and Cryomedicine, 21(4), 395–406. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/111

Issue

Vol. 21 No. 4 (2011): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 A. N. Sukach, T. D. Lyashenko

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