The Determination of Metabolic Activity of Freshly Isolated and Cryopreserved Human Fetal Liver Cells Using Alamar Blue Test

Authors

  • Yu. A. Petrenko
  • N. A. Gorokhova
  • B. P. Sandomirsky
  • A. Yu. Petrenko

Keywords:

Alamar Blue, fetal liver cells, cryopreservation, metabolic activity, fluorescence

Abstract

The possibility of using the metabolic indicator Alamar Blue (AB) for fetal liver (FL) cells viability and proliferative activity assessment before and after cryopreservation was investigated. It is shown, that AB is the integral indicator of cells metabolic activity, which reflects the plasma membrane integrity as well as glycolysis intensity and respiratory chain activity in mitochondria. The inhibitory analysis of the AB reduction by human FL cells demonstrated that the glycolysis took the significant part in the total metabolic activity of cells of embryonic origin. The results demonstrate that the AB is a highly sensitive viability test for human FL cells and it could be used for the screening of cryopreservation protocol efficiency.

Author Biographies

Yu. A. Petrenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

N. A. Gorokhova

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

B. P. Sandomirsky

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

A. Yu. Petrenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Downloads

Published

2005-12-25

How to Cite

Petrenko, Y. A., Gorokhova, N. A., Sandomirsky, B. P., & Petrenko, A. Y. (2005). The Determination of Metabolic Activity of Freshly Isolated and Cryopreserved Human Fetal Liver Cells Using Alamar Blue Test. Problems of Cryobiology and Cryomedicine, 15(4), 599–606. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1208

Issue

Vol. 15 No. 4 (2005): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).