Influence of Temperature and Ionic Strength of Medium on Surface Potential of Human Erythrocytes

Authors

  • Olga I. Gordiyenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Igor F. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo27.01.019

Keywords:

erythrocytes, cooling, surface potential, surface concentration of ions

Abstract

During freezing of cell suspensions the ionic strength of extracellular and, subsequently, intracellular media is responsible for the changes in membrane potential of cells. In this research we revealed the temperature and concentration dependencies of the surface potential of human erythrocytes during freezing of the cell suspension at the points corresponding to the values of temperature and 1:1 concentration of the electrolyte according to the phase diagram of a water-NaCl binary system. The concentrations of sodium ions on the surface of erythrocytes depending on concentration and temperature of the bulk solution were determined. It has been shown that the concentration of sodium ions on the surface of cells was 1.5 times higher than that in the bulk solution. The kink of surface potential temperature dependence for human erythrocytes for 0.15 and 0.3 M concentrations of 1:1 electrolyte within 8...12°C temperature range was of special interest. This temperature dependence feature of the surface potential calculated for the charged surface with a particular surface charge density from the purely physical considerations without taking into account the structure and composition of the surface could be one of the causes of rearrangements in membrane at these temperatures. Misbalance of electrostatic interactions on the surface of membrane outer monolayer appeared as a result of an increased concentration of counterions on the surface of cells and correspondingly the reduced surface potential might trigger the rearrangements and changes in relationships between membrane protein and lipid components.

Probl Cryobiol Cryomed 2017; 27(1): 19–28

 

Author Biographies

Olga I. Gordiyenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Low Temperature Preservation

Igor F. Kovalenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Low Temperature Preservation

References

Cevc G. Membrane electrostatics. Biochim Biophys Acta 1990; 1031(3): 311–382. CrossRef

Davydova E.V., Gordiyenko O.I. Temperature effect on erythrocyte membrane permeability for cryoprotectants with different hydrophobicities. Probl Cryobiol 2009; 19(3): 261–272.

Davydova E.V., Gordiyenko O.I. Temperature effect on erythrocyte distribution by spherical index. V.N. Karazin KhNU Biophysical Bull 2009; 2(23): 114–119.

Demchenko A.P., Mely Y., Duportail G., Klymchenko A.S. Monitoring biophysical properties of lipid membranes by environment-sensitive fluorescent probes. Biophys J 2009; 96(9): 3461–3470. CrossRef PubMed

Forte T., Leto T.L., Minetti M., Marchesi V.T. Protein 4.1. is involved in structural thermotropic transition of the red blood cell memb-rane detected by spin-labeled stearic acid. Biochemistry 1985; 24(27): 7876–7880. CrossRef PubMed

Gordiyenko O.I. Temperature effect on human erythrocyte membrane permeability for 1,2-propanediol and dimethylsulfoxide. Probl Cryobiol 2003; 13(1): 38–45.

Gordiyenko O.I., Yemets B.G., Zhilyakova T.A., Sheykin V.I. Temperature dependence of the water diffusion permeability of erythrocyte membranes in media of various ionic strengths. Biologischeskie Membrany 1985; 2(3): 310–314.

Israelachvili J. Intermolecular and surface forces. London: Academic Press; 2004.

Levtov V.A., Regirer S.A., Shadrina N.Kh. Blood rheology. Ðœoscow: Meditsina; 1982.

Mazur P., Leibo S.P., Chee E.H.Y. A two factors hypothesis of freezing injury. Cell Res 1972; 71(2): 345–355. CrossRef

Petelska A.D., Janica R., Kotynska J. et al. The effect of contrast medium SonoVue on the electric charge density of blood cells. J Membrane Biol 2012; 245(1): 15–22. CrossRef PubMed

Pogorelov A.V. Surfaces bending and membranes stability. Ðœoscow: Nauka; 1986.

Schwarz S., Haest C.W.M, Deuticke B. Extensive electroporation abolishes experimentally induced shape transformations of erythrocyte: a consequence of phospholipid symmetrization? BBA 1999; 1421(2): 361–379.

Sheetz M.P., Singer S.J. Biological membranes as a bilayer couples. A mechanism of drug erythrocyte interaction. Proc Natl Acad Sci USA 1974; 71(14): 4457–4461.

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Published

2017-03-25

How to Cite

Gordiyenko, O. I., & Kovalenko, I. F. (2017). Influence of Temperature and Ionic Strength of Medium on Surface Potential of Human Erythrocytes. Problems of Cryobiology and Cryomedicine, 27(1), 19–28. https://doi.org/10.15407/cryo27.01.019

Issue

Vol. 27 No. 1 (2017): Probl Cryobiol Cryomed

Section

Theoretical and Experimental Cryobiology

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