Duration of Whole-Body Air-Cryo-Exposure (â€“120Â°C) Affects Some Properties of Murine Erythrocytes
Keywords:air-cryo-therapy, erythrocytess, erythrocyte shape, osmotic fragility, hemolysis, mice
An influence of whole-body air-cryo-exposure (ACE) (â€“120Â°C) performed in cryochamber for experimental animals rendered on the populations, osmotic fragility and the hemolysis level in erythrocytes of peripheral blood (PB) of mice was studied. The analysis of our findings showed that the duration of one session of ACE (40, 60 and 90 sec) affected the qualitative and quantitative composition of erythrocytes, i. e.their total number in the PB was reduced, in particular on an account of discocytes, the part of reversibly modified forms (stomatocytes) was significantly increased. Whole-body ACE led to an increased osmotic fragility and elevated hemolysis of erythrocytes. An hour following the ACE we have not found any normalization in quantitative and population composition of erythrocytes of mice PB. Thus the observed effect, rendered by a whole-body cooling on the hemolysis in erythrocytes, enabled to state that the longer (60 and 90 sec) staying of the animals at â€“120Â°C had less impact on erythrocytes comparing to whole-body short-term extreme cooling of mice for 40 seconds.
Probl Cryobiol Cryomed 2017; 27(1): 29â€“40
Alekhin A.I, Denisov L.N., Iseev L.R. et al. Aerocryotherapy in current medicine: Practice Guideline. Moscow: Meditsyna; 2002.
Al-Rabeei M.A.M., Chalabov S.I., Astaeva M.D., Klichkhanov N.K. Osmotic fragility of erythrocytes and plasma memb-rane thiol groups concentration depends on duration of mild hypothermia in rats. Sovremennye Problemy Nauki i Obrazovaniya 2015; 3: 1â€“8.
Babiychuk V.G. Effect of extreme cryotherapy on morphofunctional state of central nervous and cardiovascular systems. Probl Cryobiol 2005; 15(3): 458â€“464.
Baraboy V.A., Sutkovoy D.A. Oxidative-antioxidative homeostasis in norm and pathology. Kyiv: Naukova Dumka; 1997.
Baranov A.Yu, Kidalov A.Yu. Treatment by cold. Cryomedicine. Saint-Petersburg; 1999.
Gordienko E.A., Gordienko O.I., Kovalenko I.F., Rozanov L.F. Experimental studies on the kinetics of hypotonic and acidic hemolysis of human erythrocytes by the method of small angle scattering. Probl Cryobiol 1994; 1: 32â€“39.
Zinchuk V.V. Deformability of erythrocytes: physiological aspects. Uspekhi Fiziologicheskikh Nauk 2001; 32(3): 66â€“78. PubMed
Klenov N.A., Klenov R.O. Structure, metabolism and functional activity of human erythrocytes in norm and pathology. Samara: Publishing House of Samara University; 2009.
Kudokotseva O.V., Kovalenko I.F., Lomakin I.I., Babijchuk G.A. Correction of anemia resulting from 5-fluorouracil toxic effect using cryopreserved preparations of cord blood. Probl Cryobiol Cryomed 2014; 24(4): 312â€“321. CrossRef
Lipunova E.A., Skorkina M.Yu. Physiology of blood: Monograph. Study. Belgorod: Publ. House of Belgorod State University; 2007.
Lomakin I.I., Kudokotseva O.V. General extreme aero-cryo-therapy. Provisor 2006; 3: 14â€“16.
Lomakin I.I., Kudokotseva O.V., Babijchuk V.G. Experimental cryochamber to study mechanisms of aerocryotherapy of laboratory animals. Proc. of the 6th International Scientific-Practical Conference 'Cryotherapy in Russia'. Saint-Petersburg; 2013; 36â€“43.
Lomako V.V., Kovalenko I.F., Shilo A.V. Peripheral blood erythrocytes at various types of hypothermia of homoiothermal organism. Probl Cryobiol 2012; 22(4): 398â€“409.
Moroz V.V., Golubev A.M., Afanasyev A.V. et al. Structure and function of red blood cell in norm and under critical condi-tions. General Reanimatology 2012; 8(1): 52â€“56. CrossRef
Sakhau N.R., Mirsaeva G.Kh., Kamilov F.Kh. et al. Clinical and diagnostic assessment of the state of erythrocyte membranes in patients with primary chronic pyelonephritis. Nephrology 2005; 9(1): 47â€“51.
Tadzhibova L.T., Astaeva M.D., Ismailova Zh.G. et al. Effects of dalargin on free radical processes in the blood of rats exposed to moderate hypothermia. Bul Exp Biol Med 2010; 150(9): 271â€“274.
Emirbekov E.Z., Klichkhanov N.K. Free-radical processes and state of membranes under hypothermia. â€“ Rostov-on-Don: Southern Federal University Publ. House; 2011. PubMed
Gordienko E. A., Gordienko Yu. E., Gordienko O. I. The physico-mathematical theory of human erythrocyte hypotonic hemolysis phenomenon. CryoLetters 2003; 24(4): 229â€“244.
Srour M. A., Bilto Y.Y., Juma M., Irhimeh M.R. Exposure of human erythrocytes to oxygen radicals causes loss of deformability, increased osmotic fragility, lipid peroxidation and protein degradation. Clin Hemorheol Microcirc 2000; 23(1): 13â€“21. PubMed
How to Cite
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).