Effect of Cryopreserved Cord Blood Serum on Systemic Inflammatory Responses in Experimental Cold Wound
Keywords:cryodamage, cryopreserved cord blood serum, C-reactive protein, leukogram, erythrocyte sedimentation rate, peroxidation processes
The effect of cryopreserved cord blood serum on systemic inflammatory responses following skin cryodestruction was studied. The investigation comprised the assessment of inflammation response indices: erythrocyte sedimentation rate, leukogram, amount of C-reactive protein and TBA reactive substances in blood serum after the treatment with the human cryopreserved cord blood serum of animals with cold wounds. It has been shown that cryodamage of rat skin led to an inflammation response accompanied with a two-fold erythrocyte sedimentation rate, 1.5 elevation of C-reactive protein to observation days 7 and 14, changes in leukogram; 1.4 rise in the level of peroxidation processes to day 7, 2.5 times to day 9, 1.9 times to day 14 and 1.5 times to day 21. Application of cryopreserved cord blood serum resulted in a reduced intensity of inflammation response to skin cryodestruction in experimental animals, that was confirmed by a 1.5 time decrease in the level of C-reactive protein to days 7and 9, and its normalization to day 14; 2.0 times diminishing of erythrocyte sedimentation rate to day 7 and its normalization to day 14; normalization of leukogram to day 7; 1.4 times reduction of TBA reactive product level to day 7 and its normalization to day 9.
Probl Cryobiol Cryomed 2017; 27(1): 71-80
Andreeva N.N., Solovyova T.I., Balandin M.V, Yakovlev E.I. Research activity of lipid peroxidation, antioxidant system and ultrastructure of organs in long-term of postresuscitative time. Modern Tehnol Med 2012; (2); 37â€“43.
Chevari S., Andel G., Strenger Ya. Determination of AO blood parameters. Lab Delo 1991; 10; 9â€“14.
Gulevsky A.K., Abakumova E.S., Schenyavsky I.I. Use of biologic preparations in the treatment of ischemic heart damage. Biotechnol Acta 2013; 6(2); 43â€“57.
Ischenko I.O., Tynynyka L.N., Kovalov G.A., Iefimova I.A., Sandomirsky B.P. Effect of cryopreserved cord blood serum on biochemical markers of destruction of tissues. Experimental and Clinical Medicine 2016; 70(1); 19â€“25.
Karamanos D., Karkos C., Kambaroudis A. Ðµt al. The effect of Antithrombin-III on routine hematological and biochemical parameters in an experimental animal model of skeletal muscle is-chemia-reperfusion injury. Hippokratia 2014; 18 (3); 234â€“239.
Lipina O.V., Musatova I.B., Trifonov Yu.V., Prokopyuk O.S. Pre-paration of human cord blood serum: clinical use. Svit Meditsiny i Biologii 2009; 5(4); 36â€“38.
Lucas V., Barrera R., Duque F. Ðµt al. Effect of exercise on serum markers of muscle inflammation in Spanish Greyhounds. Am J Vet Res 2015; 76 (7); 637â€“643.
Menshikov V.V., editor. Clinical laboratory analytics. Vol. II. Special analytical technologies in clinical laboratory. Moscow: Labinform, 1999.
Oleinik G.A. Pathophysiology of cold shock. Emergency Medicine 2013; 50(3); 16â€“21.
Rybolovlev Yu.R., Rybolovlev R.S. Dosage of substances for mammals on biological activity constant. Zhurnal Dokladov AN SSSR 1979; 247(6); 1513â€“1516.
Prokopyuk O.S., Lipina O.V., Musatova I.B. About clinical efficirncy of cryopreserved human cord blood serum. Probl Cryobiol 2008; 18 (4); 535â€“537.
Schiffman F.J. Pathophysiology of blood. â€“ Moscow, St. Petersburg: Benom-Nevsky Dialect, 2000. â€“ 448 p.
Ushakova T.A., Alekseeva A.A., Krutikov M.G., Bobrovnikov A.E. Inflammatory markers in the diagnosis of burn sepsis. Kambustiologiya 2015; 54(1); 84â€“91.
Watson J.D., Gifford S.M., Clouse W.D. Biochemical markers of acute limb ischemia, rhabdomyolysis, and impact on limb salvage. Semin Vasc Surg 2014; 27(3â€“4); 176â€“181.
How to Cite
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).