State of Human Cord Blood Nucleated Cells After Cryopreservation with DMSO and Antioxidants and Transfer to Conditions Simulating Physiological

Authors

  • Olena E. Makashova Department of Cryocytology
  • Oksana L. Zubova Department of Cryocytology
  • Pavlo M. Zubov Department of Cryocytology
  • Lubov O. Babiychuk Department of Cryocytology

DOI:

https://doi.org/10.15407/cryo28.01.039

Keywords:

human cord blood, nucleated cells, cryopreservation of nucleated cells, dimethylsulfoxide, antioxidant, ascorbic acid, N-acetyl-L-cysteine, glutathione

Abstract

Probl Cryobiol Cryomed 2018; 28(1): 039–043

Author Biographies

Olena E. Makashova, Department of Cryocytology

Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine,
Kharkiv, Ukraine

Oksana L. Zubova, Department of Cryocytology

Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine,
Kharkiv, Ukraine

Pavlo M. Zubov, Department of Cryocytology

Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine,
Kharkiv, Ukraine

Lubov O. Babiychuk, Department of Cryocytology

Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine,
Kharkiv, Ukraine

References

Babijchuk L.A., Makashova E.E., Zubova O.L., Zubov P.M. Evaluation of the antioxidant properties of ascorbic acid at cryopreservation of cord blood nucleated cells with DMSO. Proceeding of Conference 'Transplantation – present, past and future'; 2014 Nov 7; Kyiv, Ukraine, 2014. Cell and Organ Transplantology; 2(2): 166.

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Fry L.J., Querol S., Gomez S.G. et al. Assessing the toxic effects of DMSO on cord blood to determine exposure time limits and the optimum concentration for cryopreservation. Vox Sang 2015; 109 (2): 181–190. CrossRef PubMed

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Rushworth G.F., Megson I.L. Existing and potential therapeutic uses for N-acetylcysteine: the need for conversion to intracellular glutathione for antioxidant benefits. Pharmacol Ther 2014; 141(2): 150–159. CrossRef PubMed

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Published

2018-03-25

How to Cite

Makashova, O. E., Zubova, O. L., Zubov, P. M., & Babiychuk, L. O. (2018). State of Human Cord Blood Nucleated Cells After Cryopreservation with DMSO and Antioxidants and Transfer to Conditions Simulating Physiological. Problems of Cryobiology and Cryomedicine, 28(1), 39–43. https://doi.org/10.15407/cryo28.01.039

Issue

Vol. 28 No. 1 (2018): Probl Cryobiol Cryomed

Section

Stem Cell Day. Short Communications

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