Peculiarities of Culture and In vitro Contact Interaction of Cryopreserved Thymic Multipotent Stromal Cells and Hemopoietic Cells
DOI:
https://doi.org/10.15407/cryo28.01.005Keywords:
cryopreserved multipotent stromal cells of thymus, fetal liver cells, lymphocytes, cell culture, contact cellular interactionAbstract
The cultural properties of cryopreserved and native thymic multipotent stromal cells (MSCs) of CBA mice were investigated. These cells differentiated in osteogenic and adipogenic directions in special media. Native and cryopreserved cells showed similar properties when cultured in glass petri dishes, plastic plates and cellophane. Both native and cryopreserved MSCs of thymus formed fibroblast colonies on glass and plastic surfaces. Cell growth was observed in cellophane. The thymus MSCs were found to have a membrane affinity for thymocytes, lymphocytes of lymph nodes and fetal liver cells. This determined their ability to interact with various hematopoietic cells with the formation of associations in the form of fibroblast-lymphocytic rosettes (FLR). The most effective, i.e. with the FLR formation, contact interaction was performed when the MSCs and thymocytes were involved, although the cells of mice lymph nodes and fetal liver also formed a significant number of FLRs. MSCs cryopreservation did no affect their ability to the contact interaction, that confirmed the possibility of using the cryopreservation to create appropriate cell transplants.
Probl Cryobiol Cryomed 2018; 28(1): 005-013
References
Anderson G., Anderson K.L., Tchilian E.Z. et al. Fibroblast dependency during early thymocyte development maps to the CD25+CD44+ stage and involves interactions with fibroblast matrix molecules. Eur J Immunol 1997; 27(5): 1200–1206. CrossRef PubMed
Anderson G., Moore N.C., Owen J.J., Jenkinson E.J. Cellular interactions in thymocyte development. Annu Rev Immunol 1996; 14: 73–99. CrossRef PubMed
Chertkov I.L., Gurevich O.A. Stem hematopoietic cell and its microenvironment. Moscow: Meditsyna; 1984.
Grischenko V.I., Petrenko A.Yu., Volkova N.A., Skorobogatova N.G. Colony-forming activity of fibroblast-like precursor cells from human embryonic liver under in vitro conditions. Dopov Nac Akad Nauk Ukr 2005; 2: 128–141.
Kassis I., Vaknin-Dembinsky A., Karussis D. Bone marrow mesenchymal stem cells: agents of immunomodulation and neuroprotection. Curr Stem Cell Res Ther 2011; 6(1): 63–68. CrossRef PubMed
Le Blanc K., Mougiakakos D. Multipotent mesenchymal stromal cells and the innate immune system. Nat Rev Immunol 2012; 12(5): 383–396. CrossRef PubMed
Mouiseddine M., Mathieu N., Stefani J. et al. Characterization and histological localization of multipotent mesenchymal stromal cells in the human postnatal thymus. Stem Cells Dev 2008; 17(6); 1165–1174. CrossRef PubMed
Musina R.A., Bekchanova E.S., Sukhikh G.T. Comparison of mesenchymal stem cells obtained from different human tissues. Bull Exp Biol Med 2005; 139(4): 504–509. CrossRef PubMed
Nikolskaya E. I., Butenko G. M. Structural-functional organization of the bone marrow hematopoietic stem cells niches. Cell and Organ Transplantology 2016; 4(1): 82–100. CrossRef
Osada M., Singh V.J., Wu K. et al. Label retention identifies a multipotent mesenchymal stem cell-like population in the postnatal thymus. PLoS One 2013; 8(12): e83024. CrossRef PubMed
Pol D. Culture of cells and tissues. Moscow: Meditsyna; 1963.
Prockop D.J., Phinney D.G., Bunnell B.A. Mesenchymal stem cells: methods and protocols. Totowa, NJ: Humana Press; 2008. CrossRef
Sawada M., Nagamine J., Takeda K. et al. Expression of VLA-4 on thymocytes. Maturation stage-associated transition and its correlation with their capacity to adhere to thymic stromal cells. J Immunol 1992; 149(11): 3517–3524. PubMed
Siepe M., Thomsen A.R., Duerkopp N. et al. Human neonatal thymus-derived mesenchymal stromal cells: characterization, differentiation, and immunomodulatory properties. Tissue Eng Part A 2009; 15(7): 1787–1796. CrossRef PubMed
Skorobogatova N.G., Volkova N.A., Petrenko A.Yu. Osteogenic and adipogenic capacity of fibroblast-like progenitor cells derived from human fetal liver. Tsitologiya 2008; 4: 317–322. CrossRef
Spaggiari G.M., L. Moretta. Cellular and molecular interactions of mesenchymal stem cells in innate immunity. Immunol Cell Biol 2013; 91(1): 27–31. CrossRef PubMed
Tarasov A.I., Petrenko A.Yu., Grischenko V.I., Jones D.R.E. Post-thaw viability of human fetal liver cells of different phenotype. Probl Cryobiol 2002; 3: 36–41. Full Text
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
