Kinetics of Phase Transformations During Cooling-Warming of Saccharomyces cerevisiae Cells in Alginate-Containing Cryoprotective Media

Authors

  • Victoria L. Ponomareva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Larisa G. Kuleshova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Igor P. Vysekantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Olena S. Onasenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Olga О. Mykhailova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-8854-2872

DOI:

https://doi.org/10.15407/cryo28.03.212

Keywords:

cryopreservation, yeast, sodium alginate, viability, cryomicroscopy

Abstract

The effect of sodium alginate on the viability and kinetics of phase transformations of Saccharomyces cerevisiae cells during cryopreservation was investigated. There were determined the optimal conditions for yeast cell cryopreservation such as: the cooling rate of 1 deg/min in alginate-containing cryoprotective media (cell viability of (90.79 ± 2.01)% and (88.11 ± 1.75)% in solution of 1% sodium alginate and in combination of 5% dimethyl sulfoxide (DMSO) and 1% sodium alginate, respect tively). Using cryomicroscopy, the presence of sodium alginate in cryoprotective medium was shown to change the crystallization performance and to shift the phase transition zone towards lower temperatures. The beginning of phase transformations in the specimens, cooled in 5% DMSO solution was recorded at –8.5°C, in those, containing 1% sodium alginate and in a three-component system, comprising the combination of 1% sodium alginate and 5% DMSO, it was found at –16 and –15°С, respectively.

 

Probl Cryobiol Cryomed 2018; 28(3): 212–223

Author Biographies

Victoria L. Ponomareva, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryomicrobiology

Larisa G. Kuleshova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Low Temperature Preservation

Igor P. Vysekantsev, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryomicrobiology

Olena S. Onasenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryomicrobiology

Olga О. Mykhailova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

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Published

2018-09-27

How to Cite

Ponomareva, V. L., Kuleshova, L. G., Vysekantsev, I. P., Onasenko, O. S., & Mykhailova, O. О. (2018). Kinetics of Phase Transformations During Cooling-Warming of Saccharomyces cerevisiae Cells in Alginate-Containing Cryoprotective Media. Problems of Cryobiology and Cryomedicine, 28(3), 212–223. https://doi.org/10.15407/cryo28.03.212

Issue

Section

Theoretical and Experimental Cryobiology