Experimental Model of Skin Cryodestruction


  • Gennadiy O. Kovalov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Oleksandr O. Vlasov V.N. Karazin Kharkov National University, Kharkiv
  • Mykhailo S. Myroshnychenko Kharkiv National Medical University of the Ministry of Health of Ukraine
  • Boris P. Sandomirskiy Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv




skin cryodestruction, model, cryoprobe, temperature, tissue morphology, oxidative stress, ceruloplasmin, C-reactive protein


We suggest the in vivo experimental model of skin cryodestruction, suitable for studying the processes, occurring after skin neoplasm cryosurgery. To develop the model we have used low-temperature exposure using a cryoprobe, providing the temperature below the tissue cryoresistance threshold in the area of contact between the applicator working surface and skin. The initiation of destructive and inflammatory processes, both local and systemic, was observed in this model. Local morphological manifestations corresponded to the first phase of wound–healing process. We noted the destructive and inflammatory signs accompanied by primary necrosis (due to direct thermal damage), and a leukocyte bank and secondary necrosis (resulting from hemodynamic disorders). The reparative processes in injured tissues were manifested in developed granulation tissue. At a system level, the tissue cryodestruction was accompanied by the oxidative stress development (an increased content of lipid peroxidation products, a suppressed enzymatic component of antioxidant defense system) and an increased content of cerulloplasmin and C-reactive protein in blood serum.

Probl Cryobiol Cryomed 2019; 29(1): 088–101


Author Biographies

Gennadiy O. Kovalov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Experimental Cryomedicine

Boris P. Sandomirskiy, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Experimental Cryomedicine


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How to Cite

Kovalov, G. O., Vlasov, O. O., Myroshnychenko, M. S., & Sandomirskiy, B. P. (2019). Experimental Model of Skin Cryodestruction. Problems of Cryobiology and Cryomedicine, 29(1), 88–101. https://doi.org/10.15407/cryo29.01.088



Cryomedicine, Clinical and Experimental Transplantology