Influence of Cryopreserved Human Placental Tissue on Reparative Bone Formation in Rats with the Lower Jaw Open Fracture on Osteoporosis Background


  • Oleksii O. Likhitskyi National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • Anatoliy M. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Acadmy of Sciences of Ukraine, Kharkiv, Ukraine



cryopreserved tissue, human placenta, calcium citrate, reparative osteogenesis, mandible fracture, bone tissue metabolism, rats


The influence of calcium citrate and cryopreserved human placental tissue reparative osteogenesis in rats with an open fracture of the lower jaw on the background of osteoporosis was investigated. The studies were performed
in three groups (n = 35 each) of mature Wistar male rats (180–200 g weight): the rats with a simulated open mandibular fracture on the osteoporosis background, rats with simulated pathology, which implanted with the cryopreserved human placental tissue and the rats with a simulated pathology, which in addition to an implantation of the cryopreserved human
placental tissue were also injected with calcium citrate. Experimental osteoporosis in rats was induced by injecting of 60% solution of hydrocortisone acetate for 60 days in a dose of 50 mg/kg every 24 hours. Afterwards traumatic damage to the lower jaw was simulated. The model showed that cryopreserved placental tissue had an osteoprotective effect, which was manifested in enhancing the collagen formation processes (maximum to day 30), as well as bone mineralization, depriving the effect on osteo-destructive processes and collagen catabolism (maximum to day 14). The combined use of cryopreserved human placenta and calcium citrate had a powerful osteoprotective effect, which also developed at the early stages of experiment if compared wuith monotherapy.

Probl Cryobiol Cryomed 2019; 29(2):125–136.

Author Biography

Anatoliy M. Goltsev, Institute for Problems of Cryobiology and Cryomedicine of the National Acadmy of Sciences of Ukraine, Kharkiv, Ukraine

Department of cryopathophysiology and immunology


Arosarena O, Ducic Y, Tollefson TT. Mandible fractures: discussion and debate. Facial Plast Surg Clin North Am. 2012; 20(3): 347-63. CrossRef

Bedyk ОV, Polishchuk SS, Shuvalov SМ, inventors; National Pirogov Memorial Medical University, Vinnytsya, assignee. [Method of modeling of standardized fractures of the mandible in the experiment.] Patent of Ukraine 63813U. 2011 October 25. Ukrainian.

Dillon JK, Christensen B, McDonald T, et al. The fi nancial burden of mandibular trauma. J Oral Maxillofac Surg. 2012; 70(9): 2124-34. 4. Galytska AK, Bortkevich OP. [Application of calcium medications for prophylaxis and treatment of osteoporosis]. Ukrayinsʹkyy revmatolohichnyy zhurnal. 2002; 10(4): 37-40. Russian. CrossRef

Goltsev AN, Yurchenko TN, editors. [Placenta: cryopreservation, clinical use: monograph (In:)]. Kharkov: Brovin AV, 2013. 318 р. Russian.

Grimm WD, Ploger M, Schau I. et al. Implex, three-dimensional reconstruction of critical size defects following delayed implant placement using stem cell-containing subepithelial connective tissue graft and allogenic human bone blocks for horizontal alveolar bone augmentations case report as proof of clinical study principles. Meditsinskiy vestnik Severnogo Kavkaza. 2014; 9, 2(34): 131-3. CrossRef

Ibrahim N, Mohamad S, Mohamed N, et al. Experimental fracture protocols in assessments of potential agents for osteoporotic fracture healing using rodent models. Curr Drug Targets. 2013; (14): 1642-50. CrossRef

Kopchak AV. [Direct and distant results of surgical treatment of patients with traumatic fracture of the mandible]. Klinichna khirurhiya. 2014; (1): 56-60. Ukrainian.

Krivoruchko IA, Goltsev KA, Grishchenko VI, et al. [Combined treatment of acute purulent peritonitis with cryopreserved cord blood]. Kharkiv surgical school. 2011; 51(6): 47-52. Russian.

Levitsky AP, Makarenko OA, Denga OV. [Experimental methods for the study of osteogenesis stimulants: a method. recommendations]. Kyiv: GFTs, 2005. 30 р. Russian.

Levitsky AP, Makarenko OA, Khodakov IV, et al. [An enzymatic method for assessing the state of bone tissue]. Odesʹkyy medychnyy zhurnal. 2006; (3): 17-21. Ukrainian.

Liu W, Morschauser A, Zhang X, et al. Human placenta-derived adherent cells induce tolerogenic immune responses. Clin Transl Immunology. 2014; 3(5): 14. CrossRef

Mamonov RO, Illienko NA. [Biochemical changes in serum of patients with fractures of the mandible]. Ukrayinsʹkyy naukovomedychnyy molodizhnyy zhurnal 2013; 2(2): 264. Ukrainian.

Menshikova VV. [Laboratory research methods in the clinic: a Handbook]. Moskow.: Meditsina, 1987. 368 р. Russian.

Robbins JA, Aragaki A, Crandall CJ, et al. Women's Health Initiative clinical trials: interaction of calcium and vitamin D with hormone therapy. Menopause. 2014; (21): 116-23. CrossRef

Sharaev PN, Sakhabutdinova EP, Lekomtseva OI, et al. [Determination of free and peptide-bound hydroxyproline in serum]. Klinicheskaya laboratornaya diagnostika. 2009; (1): 7-9. Russian.

Shevchenko NO, Somova KV, Volina VV, et al. [Dynamics of activity and duration of functioning of cryopreserved cryoextract, cells and fragments of placenta in the organism of experimental animals]. Morphologia [Internet]. 2016; 10(2): 93-8. [Cited 01.01.2019] Available from: view/139666/136647. CrossRef

Sikora VZ, Pogorelov MV, Bumeister VI, et al. [Biochemical parameters of blood in diff erent terms of reparative osteogenesis]. Problemy, dostizheniya i perspektivy razvitiya medikobiologicheskikh nauk i prakticheskogo zdravookhraneniy Trudy Krymskogo gosudarstvennogo meditsinskogo universiteta im SI Georgiyevskogo. 2007; 143(Pt.4): 84-6. Ukrainian.

Silini AR, Cargnoni A, Magatti M, et al. The long path of human placenta, and its derivatives, in regenerative medicine. Biotechnology. 2015; 19(3): 162. CrossRef

Sorokin BV, Kostenko VO. [Alterations of components of organic bone matrix in rats under modeled osteoporosis and chronic sodium nitrate intoxication]. Visnyk VDNZU «Ukrayinsʹka medychna stomatolohichna akademiya». 2013; 42(2): 220-4. Ukrainian.

Stefanov OV. [Preclinical research of drugs: a method. ecommendations]. Kyiv: Avicenna, 2001. 528 p. Ukrainian.

Tai V, Leung W, Grey A, et al. Calcium intake and bone mineral density: systematic review and meta-analysis. BMJ [Internet]. 2015; (351): 4183. [Cited 01.01.2019] Available from: https:// CrossRef

Tu KN, Lie JD, Wan CKV, et al. Osteoporosis: A Review of Treatment Options. PMC. 2018; 43(2): 92-104.




How to Cite

Likhitskyi, O. O., & Goltsev, A. M. (2019). Influence of Cryopreserved Human Placental Tissue on Reparative Bone Formation in Rats with the Lower Jaw Open Fracture on Osteoporosis Background. Problems of Cryobiology and Cryomedicine, 29(2), 125–136.



Cryomedicine, Clinical and Experimental Transplantology