Influence of Freeze-Thawing with DMSO on Integrity and Osmotic Fragility of Erythrocyte from Different Mammalian Species
Keywords:mammal erythrocytes, hemolysis, dimethyl sulfoxide, cryopreservation, osmotic fragility
In the research there was studied the hemolysis level of bovine, equine, and rabbit erythrocytes in different hypotonic media after incubation and freeze-thawing with 10% dimethyl sulfoxide (DMSO) solution. It was shown, that intact erythrocytes of all the examined animals demonstrated the similar osmotic stability in hypotonic conditions, and DMSO in the stated concentration does not significantly affect this parameter and red blood cell integrity. The use of 10% DMSO during freeze-thawing without washing-out the cryoprotectant allows to preserve up to 80% erythrocytes, however, the process of cryoprotectant removal reduces this index.
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Copyright (c) 2020 P. Yu. Ulizko, G. F. Zhegunov, O. N. Denisova
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