Study of Protective Effect of Synthetic Neuropeptide Dalargin Under Cold Stress
DOI:
https://doi.org/10.15407/cryo29.03.246Keywords:
neuropeptides, dalargin, hypothermia, cold stress, apoptosis, stress-tread effectAbstract
The effect of the neuropeptide dalargin, which is a synthetic analogue of leu-enkephalins, on the negative processes caused by cold stress, which occur when artificial hypothermia is used in vivo, was studyd. It was shown that dalargin prevents the development of apoptotic processes in the cells of homeothermic animals after cold stress, which manifested itself in a decrease in the degree of DNA fragmentation. In the kidneys and liver of experimental animals subjected to cooling, this attribute of apoptosis and cell necrosis was not detected, and in the intestinal mucosa and spleen the level of DNA fragmentation as a result of cold stress is statistically significant. The preliminary injection of dalargin to rats reduces the degree of DNA fragmentation to values that occurred in animals that were not exposed to cold stress. It is also shown that dalargin prevents the development of adrenal hypertrophy in experimental animals, reduces the degree of involution of the thymus and spleen, and reduces the severity of cold stress-induced ulcerogenesis. The simultaneous injection to animals of an opiate receptor antagonist naloxone eliminated the protective effect of an opiate receptor agonist dalargin.
Probl Cryobiol Cryomed 2019; 29(3): 246–254.
References
Ado AD, Ishimova LM, editors. [Pathological physiology], 2nd ed. Moscow: Medicine, 1980. 520 p. Russian.
Borlongan CV, Wang Y, Su TP. Delta opioid peptide (D-Ala 2, D-Leu 5) enkephalin: linking hibernation and neuroprotection. Front Biosci J. [Internet]. 2004 [cited Jan 15 2019]; (9): 3392-8. Available from: http://www.bioscience.org/2004/v9/af/1490/list.htm CrossRef
Fleck CC. Modulation of apoptotic pathways in intestinal mucosa during hibernation. Am J Physiol Regul Integr Comp Physiol. 2005; 289(2): R586-R595. CrossRef
Fu D, Liu H, Li S, Chen L, Yao J. Antioxidative and antiapoptotic effects of delta-opioid peptide [D-Ala2, D-Leu5] enkephalin on spinal cord ischemia-reperfusion injury in rabbits. Front Neurosci. [Internet]. 2017 [cited Jul 23 2018]; 11:603. Available from: https://www.frontiersin.org/articles/10.3389/fnins.2017.00603/full CrossRef
Gulevsky AK, Akhatova YuS, Shchenyavsky II Features of apoptosis, induced by temperature reduction. Probl Cryobiol Cryomed. 2017; 27(2): 97-109. CrossRef
Hendriks KDW, Lupi E, Hardenberg MC, Hoogstra-Berends F. Differences in mitochondrial function and morphology during cooling and rewarming between hibernator and non-hibernator derived kidney epithelial cells. Sci Rep. [Internet]. 2017 [cited July 23 2018]; 7(1): 15482. Available from: https://www.nature.com/articles/s41598-017-15606-z. CrossRef
Himms-Hagen J. Neural and hormonal responses to prolonged cold exposure. In: Fregly M, Blatteis C, editors. Adaptation to the environment. Handbook of Physiology. New York: Oxford University Press; 1996. p. 439-80.
Ignat'ev DA, Kolaeva SG, Kramarova LI, Kravchenko II. [Hypothermic effect on mice of a 1-10 kD fraction of the small intestine of the hibernating suslik Citellus undulatus during hypoxia and hypercapnia]. Zh Evol Biokhim Fiziol. 1989; 25( 3): 318-23. Russian.
Komarevtseva IA, Popov EN, Komarevtsevа КV, Zaika ÐV. [The promotor of apoptosis gen Ñ€53 and antyapoptotic gen Bcl-2 in cell proliferation]. Likarska sprava. 2001; (4): 115-8. Russian.
Lishmanov IuB, Maslov LN, Naryzhnaia NV et al. [Endogenous opioid system as a mediator of acute and long-term adaptation to stress. Prospects for clinical use of opioid peptides]. Vestn Ross Akad Med Nauk. 2012; (6): 73-82. Russian.
Liu H, Chen B, Li S, Yao JJ. Dose-dependent neuroprotection of delta-opioid peptide [D-Ala(2), D-Leu(5)] enkephalin on spinal cord ischemia-reperfusion injury by regional perfusion into the abdominal aorta in rabbits. Vasc Surg. 2016; 63(4):1074-81. CrossRef
Liu H, Chen B, Zhang Y, et al. Protective effect of delta opioid agonist [D-Ala2, D-Leu5] enkephalin on spinal cord ischemia reperfusion injury by regional perfusion into abdominal aorta in rabbits. J Neurosci Lett. 2015; 584:1-6. CrossRef
Lycheva NA, Kiselev VI, Shakhmatov II, Vdovin VM. [Contribution stressors of different nature in the formation of a hemostatic response reaction of the organism under the influence of general hypothermia]. Fundamental research. 2014; (7, Pt 1): 106-10. Russian.
Mayahi MTJ, Klichkhanov NK [Effect of dalargin on pituitary adrenal and pituitary-thyroid endocrine complex hormones content in the blood of rats during hypothermia]. Izvestia of Samara Scientific Center of the Russian Academy of Sciences. 2012; 14(5): 273-7. Russian.
Quinones QJ, Ma Q, Zhang Z, et al. Organ protective mechanisms common to extremes of physiology: a window through hibernation biology. Integr Comp Biol. 2014;54(3):497-515. CrossRef
Selye H. Stress without distress, New York: Signet, 1974. 116 p.
Stefanova OV, editors. [Preclinical research of medicinal products (methodical recommendations)]. Kyiv: Avitsenna, 2002. 527 p. Ukrainian.
Stocks JM, Taylor NA, Tipton MJ, Greenleaf JE. Human physiological responses to cold exposure. Aviat Space Environ Med. 2004; 75(5): 444-57.
Tamura Y, Monden M, Shintani M, et al. Neuroprotective effects of hibernation-regulating substances against low-temperature induced cell death in cultured hamster hippocampal neurons. Brain Res. 2006; 1108(1): 107-16. CrossRef
Yao LL, Wang YG, Cai WJ, et al. Survivin mediates the anti-apoptotic effect of delta-opioid receptor stimulation in cardiomyocytes. J Cell Sci. 2007; 120(5): 895-907. CrossRef
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