Effect of Cryopreserved Cord Blood Serum on Reparation Processes in Rat Brain Tissue With Acute Focal Cerebral Ischemia
Keywords:acute focal cerebral ischemia, necrosis, reparation, cryopreserved cord blood serum
The research was performed to investigate the possibility of stimulating reparative processes in rats with acute focal cerebral ischemia (AFCI) byÂ administering the cryopreserved cord blood serum (CCBS) of human. A comparative morphometric analysis of the structural components of the cerebral cortex sensor-motor areaÂ was conducted in dynamics of treatment. The development of a potent neurotoxic effect during the AFCI was confirmed by qualitative and quantitative indices, in particular by the 4.57 times increase ofÂ alterational andÂ in 3.62 times of the perineural satellite indices relative to the control. Degenerative processes were accompanied with powerful microcirculatory changes associated with the brain tissue swelling, as evidenced by aÂ rise inÂ average area of perivascular spaces by 45 times versus intact animals and pronounced secondary spasm of the vessels. The use of CCBS stimulated reparative processes in the necrotic zone by increasing the capillary density by 21.8% if compared to intact animals and preventedÂ the development of the blood-brain barrier andÂ membrane-receptor complex dysfunction.
Probl Cryobiol Cryomed 2019; 29(3):Â 277â€“290.
Adini A, Adini I, Ghosh K, et al. The stem cell marker prominin-1/CD133 interacts with vascular endothelial growth factor and potentiates its action. Angiogenesis. 2013; 16 (2): 405-16. CrossRef
Benjamin EJ, Blaha MJ, Chiuve SE, et al. Heart disease and stroke statistics - 2017 update: a report from the American Heart Association. Circulation. [Internet] 2017 [cited 2017 Jan 25]; 135(10): e146-e603. Available from: https://www.ahajournals.org/doi/pdf/10.1161/CIR.0000000000000485.
Bok S, Kim YE, Woo Y. Hypoxia-inducible factor-1Î± regulates microglial functions affecting neuronal survival in the acute phase of ischemic stroke in mice. Oncotarget. 2017; 8 (67): 111508- 21. CrossRef
Brandao D, Costa C, Canedo A, Ðµt al. Endogenous vascular endothelial growth factor and angiopoietin-2 expression in critical limb ischemia. Int. Angiol. 2011; 30 (1): 25-34.
Budantsev AYu, editor. [Stereotaxic atlas of the rat brain (frontal sections)]. Pushchino: Analiticheskaya mikroskopiya; 2002. p. 7-15. Russian.
Buzzia M, Versurab P, Grigoloc B, et al. Comparison of growth factor and interleukin content of adult peripheral blood and cord blood serum eye drops for cornea and ocular surface diseases. Transfus Apher Sci. 2018; 57: 549-55. CrossRef
Castellano JM, Mosher KI, Abbey RJ, et al. Human umbilical cord plasma proteins revitalize hippocampal function in aged mice. Nature. 2017; 544(7651): 488-92. CrossRef
Chernii VI, Kolesnikov AN, Gorodnik GA., Ðµt al. [Assessment of the risk of thromboembolic complications and the effectiveness of various prevention methods in patients in critical condition.] Praktichna angÑ–ologÑ–ya. 2008; 7-8: 59-65. Russian.
DÃ¡valos A, Avarez-SabÃn J, Castillo J, et al. Citicoline in the treatment of acute ischaemic stroke: an international, randomised, multicentre, placebo-controlled study (ICTUS trial). Lancet. 2012; 380(9839): 349-57. CrossRef
Ehrhart J, Sanberg PR, Garbuzova-Davis S. Plasma derived from human umbilical cord blood: Potential cell-additive or cellsubstitute therapeutic for neurodegenerative diseases. J Cell Mol Med. 2018; 22: 6157-66. CrossRef
Eve DJ, Ehrhart J, Zesiewicz T, et al. Plasma derived from human umbilical cord blood modulates mitogen-induced proliferation of mononuclear cells isolated from the peripheral blood of ALS patients. Cell Transplant. 2016; 25: 963-71. CrossRef
Fu FW, Rao J, Zheng YY. Ischemic stroke in patients with POEMS syndrome: a case report and comprehensive analysis of literature. Oncotarget. 2017; 8 (51): 89406-24. CrossRef
Jia L, Zhou X, Huang X, et al. Maternal and umbilical cord serum- derived exosomes enhance endothelial cell proliferation and migration. FASEB J. 2018; 32(8): 4534-43. CrossRef
Katsimpardi L, Litterman NK, Schein PA, et al. Vascular and neurogenic rejuvenation of the aging mouse brain by young systemic factors. Science. 2014; 344(6184), 630-4. CrossRef
Kim Y-M, Jung M-H, Song H-Y, et al. Ex vivo expansion of human umbilical cord blood-derived T-lymphocytes with homologous cord blood plasma. Tohoku J Exp Med. 2005; 205: 115-22. CrossRef
Kolesnik VV. Experimental thromboembolic stroke in Wistar rats as an alternative pathophysiological model of acute disorders of microcirculation in ischemic type. Pathologia. 2011; 8(1): 56-9. Russian.
Lam AC, Li K, Zhang XB, et al. Preclinical ex vivo expansion of cord blood hematopoietic stem and progenitor cells: duration of culture; the media, serum supplements, and growth factors used; and engraftment in NOD/SCID mice. Transfusion. 2001; 41: 1567-76. CrossRef
Li M, Zhang Y, Feurino LW, et al. Interleukin-8 increases vascular endothelial growth factor and neuropilin expression and stimulates ERK activation in human pancreatic cancer. Cancer Sci. 2008; 99: 733-7. CrossRef
Lin W, Hsuan Y, Lin M, et al. Human umbilical cord mesenchymal stem cells preserve adult newborn neurons and reduce neurological injury after cerebral ischemia by reducing the number of hypertrophic microglia/macrophages. Cell Transplantation. 2017; 26(11), 1798-810. CrossRef
Martin D, Galisteo R, Gutkind JS. CXCL8/IL8 stimulates vascular endothelial growth factor (VEGF) expression and the autocrine activation of VEGFR2 in endothelial cells by activating NFkappaB through the CBM (Carma3/Bcl10/Malt1) complex. J Biol Chem. 2009; 284: 6038-42. CrossRef
Min K, Song J, Lee JH, et al. Allogenic umbilical cord blood therapy combined with erythropoietin for patients with severe traumatic brain injury: three case reports. Restor Neurol Neurosci. 2013; 31: 397-410.
Miziuk VM. [Endothelial dysfunction and means of its correction in patients with hypertension and concomitant heart failure.] Buk. Med. Herald. 2014; 18(2): 66-8. Ukrainian.
Stefanov OV, Editor. [Preclinical studies of drugs. Guidelines.] Kyiv: Avitsenna; 2001. 528 p. Ukrainian.
Toshkezi G, Kyle M, Longo SL, et al. Brain repair by hematopoietic growth factors in the subacute phase of traumatic brain injury. J Neurosurg [Internet]. 2018 Jan 26 [cited 2018 Dec 27]. Available from:https://thejns.org/view/journals/j-neurosurg/129/5/article-p1286.xml CrossRef
Trifonov VYu, Prokopyuk VYu, Zaychenko AV. Cryopreserved cord blood serum for reproductive function restoration during antiphospholipid syndrome. Problems of Ð¡ryobiology. 2011; 21(1): 75-84.
Vvedenskiy BP, Kovalev GA, Tynynyka LN, et al. [Cryopreserved cord blood serum in treatment of destructive and dystrophic processes in joints.] Bulletin of Urgent and Recovery Medicine. 2012; 13(1): 41-3. Russian.
Yoo J, Kim H, Seo J, et al. Therapeutic effects of umbilical cord blood plasma in a rat model of acute ischemic stroke. Oncotarget. 2016; 7(48): 79131-40. CrossRef
How to Cite
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).