Impact of Lyophilisation on Integrity of Structural and Functional Characteristics of Human Cord Blood Leukoncentrate
Keywords:lyophilisation, human cord blood leukoconcentrate, colony forming units, hematopoietic stem cells, T-regulatory cells
This paper presents the investigations on the impact of lyophilisation conditions on structural and functional characteristics of human cord blood leukoconcentrate (HCBL) such as: the number and viability, cytomorphological indices, content of hematopoietic stem (CD34+) and T-regulatory cells (CD4+CD25high), hematopoietic colony forming units in culture (CFUc). Four regimens, including two programs with and without 3.5% DMSO were used for hcbl cell lyophilisation. The programs diï¬€ered in temperature value and duration wherein the primary drying proceeded. When using the ï¬rst program, the cells were dried for 10 hrs (â€“28ÂºÐ¡), and 9 hrs (â€“18ÂºÐ¡) for the second one. The designed method for HCBL lyophilisation, suggested the use of the DMSO-free ï¬rst program, ensured the cell preservation in a heterogeneous population of cord blood according to all the studied characteristics.
Probl Cryobiol Cryomed 2019; 29(4):Â 332-343
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