Histology of cryopreserved neonatal rat testes after intratesticular allotransplantation

Authors

  • Bo Deng Henan University of Science and Technology, Luoyang, PRС
  • Vitaliy Kireev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Kristina Melentyeva SI ‘I.I. Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine’, Kharkiv
  • Igor Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Oleksandr Pakhomov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo30.01.034

Keywords:

testis, intratesticular allotransplantation, cryopreservation, immune suppression, immune privilege

Abstract

Cryopreservation and transplantation of testicular fragments and cells are utilized in establishing the germplasm bank of endangered species, line-breeding of animals. Moreover, the transplantation of testicular tissue is involved into investigation of the inborn and acquired defects of testicular tissue. Thus the improvement of cryopreservation and transplantation methods is of great interest. Here, we have compared the findings of histological examination of allotransplanted native and cryopreserved neonatal testis. It has been shown that the usage of DMEM/F12 supplemented with 5 mg/ml bovine serum albumin, 0.05 M sucrose, 20 mM Hepes, 1.5 M dimethylsulfoxide as a cryoprotectant, controlled cooling, preservation at -196°C, water bath warming, followed by intratesticular allotransplantation of neonatal rat testis results in the development of testicular interstitium and spermatogenic epithelium of donor organs into recipient body.

 

Probl Cryobiol Cryomed 2020; 30(1): 34-46

Author Biographies

Vitaliy Kireev , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department for the Low Temperature Cryopreservation

Igor Kovalenko , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department for the Low Temperature Cryopreservation

Oleksandr Pakhomov , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryoendocrinology  

References

Baert Y, Van Saen D, Haentjens P, et al. What is the best cryopreservation protocol for human testicular tissue banking? Hum Reprod. 2013; 28(7): 1816-26. CrossRef

Chapman LE. Xenotransplantation, xenogeneic infections, biotechnology, and public health. Mt Sinai J Med. 2009 Oct;76(5):435-41. CrossRef

Chen Q, Deng T, Han D. Testicular immunoregulation and spermatogenesis. Semin Cell Dev Biol. 2016; 59: 157-65. CrossRef

Deng B, Bondarenko T, Pakhomov O. Changes in sexual behavior of orchidectomized rats under influence of allotransplantation of testicular interstitial cell suspension. Cell Transplant. 2017;26(5):795-803. CrossRef

Dohle GR. Male infertility in cancer patients: Review of the literature. Int J Urol. 2010; 17(4): 327-31. CrossRef

Geens M, De Block G, Goossens E, et al. Spermatogonial survival after grafting human testicular tissue to immunodeficient mice. Hum Reprod. 2006;21(2):390-6. CrossRef

Geens M, Goossens E, De Block G, et al. Autologous spermatogonial stem cell transplantation in man: current obstacles for a future clinical application. Hum Reprod Update. 2008; 14(2): 121-30. CrossRef

Geens M, Van de Velde H, De Block G, et al. The efficiency of magnetic-activated cell sorting and fluorescence-activated cell sorting in the decontamination of testicular cell suspensions in cancer patients. Hum Reprod. 2007 Mar;22(3):733-42. CrossRef

Gies I, De Schepper J, Tournaye H. Progress and prospects for fertility preservation in prepubertal boys with cancer. Curr Opin Endocrinol Diabetes Obes. 2015 ;22(3):203-8. CrossRef

Gupta AA, Donen RM, Sung L, et al. Testicular Biopsy for Fertility Preservation in Prepubertal Boys with Cancer: Identifying Preferences for Procedure and Reactions to Disclosure Practices. J Urol. 2016 ;196(1):219-24. CrossRef

Holoch P, Wald M. Current options for preservation of fertility in the male. Fertil Steril. 2011 ;96(2):286-90. CrossRef

Jahnukainen K, Ehmcke J, Hergenrother SD, Schlatt S. Effect of cold storage and cryopreservation of immature non-human primate testicular tissue on spermatogonial stem cell potential in xenografts. Hum Reprod. 2007; 22(4): 1060-7. CrossRef

Jahnukainen K, Ehmcke J, Söder O, Schlatt S. Clinical potential and putative risks of fertility preservation in children utilizing gonadal tissue or germline stem cells. Pediatr Res. 2006; 59(4 Pt 2): 40R-47R. CrossRef

Júnior AM, Arrais CA, Saboya R, et al. Neurotoxicity associated with dimethylsulfoxide-preserved hematopoietic progenitor cell infusion. Bone Marrow Transplant. 2008;41(1):95-6. CrossRef

Keros V, Rosenlund B, Hultenby K, et al. Optimizing cryopreservation of human testicular tissue: comparison of protocols with glycerol, propanediol and dimethylsulphoxide as cryoprotectants. Hum Reprod. 2005; 20(6): 1676-87. CrossRef

Kirpatovskii VI, Efremov GD, Frolova EV. Ectopic organogenesis after allotransplantation of freshly removed or cryopreserved neonatal testicle under the renal capsule in rats. Bull Exp Biol Med. 2018;166(2): 268-273. CrossRef

Krausz C, Forti G. Sperm cryopreservation in male infertility due to genetic disorders. Cell Tissue Bank. 2006; 7(2): 105-12. CrossRef

Kvist K, Thorup J, Byskov AG, et al. Cryopreservation of intact testicular tissue from boys with cryptorchidism. Hum Reprod. 2006; 21(2): 484-91. CrossRef

Mattawanon N, Spencer JB, Schirmer DA 3rd, Tangpricha V. Fertility preservation options in transgender people: A review. Rev Endocr Metab Disord. 2018; 19(3): 231-42. CrossRef

Mueller DL. Mechanisms maintaining peripheral tolerance. Nat Immunol. 2010; 11(1): 21-7. CrossRef

Paduch DA, Bolyakov A, Cohen P, Travis A. Reproduction in men with Klinefelter syndrome: the past, the present, and the future. Semin Reprod Med. 2009; 27(2): 137-48. CrossRef

Petrushko M.P. [Current state of cryopreservation of reproductive cells and embryos (According to the scientific report at the meeting of the Presidium of NAS of Ukraine, May 17, 2017)] Visn. Nac. Acad. Nauk Ukr. 2017; (7): 44-53. Ukrainian. CrossRef

Picton HM, Wyns C, Anderson RA, et al. ESHRE task force on fertility preservation in severe diseases. A European perspective on testicular tissue cryopreservation for fertility preservation in prepubertal and adolescent boys. Hum Reprod. 2015;30(11):2463-75. CrossRef

Rendtorff R, Hohmann C, Reinmuth S, et al. Hormone and sperm analyses after chemo- and radiotherapy in childhood and adolescence. Klin Padiatr. 2010; 222(3): 145-9. CrossRef

Sabanegh ES Jr, Ragheb AM. Male fertility after cancer. Urology. 2009; 73(2): 225-31. CrossRef

Sánchez-Salinas A, Cabañas-Perianes V, Blanquer M, et al. An automatic wash method for dimethyl sulfoxide removal in autologous hematopoietic stem cell transplantation decreases the adverse effects related to infusion. Transfusion. 2012;52(11):2382-6. CrossRef

Schlatt S, Honaramooz A, Boiani M, et al. Progeny from sperm obtained after ectopic grafting of neonatal mouse testes. Biol Reprod. 2003;68(6):2331-5. CrossRef

Schlatt S, Kim SS, Gosden R. Spermatogenesis and steroidogenesis in mouse, hamster and monkey testicular tissue after cryopreservation and heterotopic grafting to castrated hosts. Reproduction. 2002 ; 124(3): 339-46. CrossRef

Schuppe HC, Meinhardt A, Allam JP, et al. Chronic orchitis: a neglected cause of male infertility? Andrologia. 2008; 40(2): 84-91. CrossRef

Shu Z, Heimfeld S, Gao D. Hematopoietic SCT with cryopreserved grafts: adverse reactions after transplantation and cryoprotectant removal before infusion. Bone Marrow Transplant. 2014;49(4):469-76. CrossRef

Simpson E. A historical perspective on immunological privilege. Immunol Rev. 2006; 213: 12-22. CrossRef

Suhag V, Sunita BS, Sarin A, et al. Fertility preservation in young patients with cancer. South Asian J Cancer. 2015; 4(3): 134-9. CrossRef

Woelders H, Windig J, Hiemstra SJ. How developments in cryobiology, reproductive technologies and conservation genomics could shape gene banking strategies for (farm) animals. Reprod Domest Anim. 2012; 47 (4): 264-73. CrossRef

Yildiz C, Mullen B, Jarvi K, et al. Effect of different cryoprotectant agents on spermatogenesis efficiency in cryopreserved and grafted neonatal mouse testicular tissue. Cryobiology. 2013; 67: 70-5. CrossRef

Yokonishi T, Ogawa T. Cryopreservation of testis tissues and in vitro spermatogenesis. Reprod Med Biol. 2016; 15: 21-8. CrossRef

Zhao S, Zhu W, Xue S, Han D. Testicular defense systems: immune privilege and innate immunity. Cell Mol Immunol. 2014; 11(5): 428-37. CrossRef

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Published

2020-03-28

How to Cite

Deng , B., Kireev , V., Melentyeva , K., Kovalenko , I., & Pakhomov , O. (2020). Histology of cryopreserved neonatal rat testes after intratesticular allotransplantation. Problems of Cryobiology and Cryomedicine, 30(1), 34–46. https://doi.org/10.15407/cryo30.01.034

Issue

Vol. 30 No. 1 (2020): Probl Cryobiol Cryomed

Section

Theoretical and Experimental Cryobiology

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Copyright (c) 2020 Bo Deng , Vitaliy Kireev , Kristina Melentyeva , Igor Kovalenko , Oleksandr Pakhomov

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