Impact of Storage Temperature Regimens and Protective Media Composition on Rabies Virus CVS Strain Preservation
DOI:
https://doi.org/10.15407/cryo30.02.148Keywords:
rabies virus, cell culture, long-term storage, protective media, virus preservation, virus infectious activityAbstract
The impact of protective media supplemented with sucrose, glycerol, and maltose on the rabies virus CVS strain preservation during storage at 37, 5, –20, –80, and –196ºC has been studied. The temperature regimen of 37ºC was found as not suitable for virus storage, but temperature of 5ºC could be used for a short-term storage in corresponding protective medium in case of production need. It was shown that storage at –80 and –196ºC in DMEM-based preservation media containing 0.5% fetal bovine serum, supplemented with either 5% sucrose, or 5% glycerol, or 5% maltose, or sucrose and glycerol mixture provided high levels of the rabies virus CVS strain infectious activity within 24 months (observation period). At –20ºC, in a growth medium with sucrose and mixture of sucrose and glycerol, after 6 months 79–82% of the initial virus activity were preserved, and after 12 months that was 69–72%. Upon reaching –80ºC and during subsequent storage of virus samples at this temperature in the studied preservation media, the secondary crystallization of concentrated dissolved substances was prolonged.
Probl Cryobiol Cryomed 2020; 30(2): 148–157
References
ATCC. Virology guide. Tips and techniques for propagating virus in tissue culture and embryonated chicken eggs. [Internet]. Manassas: American Type Culture Collection; 2016. 32 p. [Cited 25.09.18] Available from: https://www.atcc.org/~/media/PDFs/Culture%20Guides/Virology_Guide.ashx
Atramentova LA, Utevska OM. [Statistical methods in biology]. Horlivka: Likhtar, 2008. 248 p. Russian.
Aubert MFA. Methods for the calculation of titers. In: Meslin FX, Kaplan MM, Koprowski H, editors. Laboratory techniques in rabies. 4 th ed. Geneva: World Health Organization; 1996. P. 445-59.
Belous AM, Grishchenko VI. [Cryobiology]. Kyiv: Naukova dumka; 1994. 432 p. Russian.
Burkova VV, Vysekantsev IP, Lavrik AA. [Preservation of infectious activity of rabies virus industrial strains stored at various temperatures]. Zhivye biokosnye sist [Internet]. 2014 [cited 01.10.19]; (9):1-11. Available from: http://www.jbks.ru/archive/issue-9/article-23. Russian.
Cardoso FMC, Petrovajová D, Horňáková T. Viral vaccine stabilizers: status and trends. Acta Virol. 2017; 61(3): 231-9. CrossRef
Costa EC, Teixeira MFS, Aguiar TDF, et. al. Rabies virus viability after short-term cryopreservation using cryoprotectant agents. Rev Inst Adolfo Lutz. 2011; 70: 106-12
Gould EA. Methods for long-term virus preservation. Mol Biotechnol. 1999; 13(1): 57-66. CrossRef
Hooper DC. Rabies virus. In: Detrick B, Schmitz JL, Hamilton RG, editors. Manual of Molecular and Clinical Laboratory Immunology. 8 th ed. Washington, DC: ASM Press; 2016. p. 665-73. CrossRef
Leibo SP, Mazur P. The role of cooling rates in low-temperature preservation. Cryobiology. 1971; 8(5): 447-52. CrossRef
Mazur P. Causes of injury in frozen and thawed cells. Fed Proc. 1965; 24(1): 175-82. PubMed3
Mazur P. Cryobiology: the freezing of biological systems. Science. 1970; 168(3934): 939-49. CrossRef
Sergeev GB, Batiuk VA. [Cryochemistry]. Moscow: Chemistry; 1978. 296 p. Russian.
Varianytsia VV, Vysekantsev IP. Storage methods of complex RNA viruses. Probl Cryobiol Cryomed. 2017; 27(4): 287-95. CrossRef
Varianytsia VV, Vysekantsev IP. Infectious activity of L. Pasteur rabies virus vaccine strain frozen-dried in various protective media and then stored at various temperatures. Probl Cryobiol Cryomed. 2018; 28(4): 333-42. CrossRef
World Health Organization Expert Committee on Biological Standardization. Recommendations for inactivated rabies vaccine for human use produced in cell substrates and embryonated eggs. In: WHO Technical Report Series - 941. Fifty-sixth report. Geneva: WHO; 2007. p. 83-132.
World Health Organization. Rabies vaccines: WHO position paper, April 2018 - recommendations. Vaccine. 2018; 36(37): 5500-3. CrossRef
World Health Organization. WHO expert consultation on rabies: third report. Geneva: WHO; 2018. 184 p.
Zhegunov GF, Nardid OA, editors. [Fundamentals of cryobiology and cryomedicine: the textbook for students of biology and medical]. Kharkiv:Brovin AV; 2019. 614 p. Russian.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 Viktoriia V. Varianytsia , Igor P. Vysekantsev
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
