Influence of Hypothermia and Cell Therapy on Structural Changes in Brain Tissues of Rats with Dyscirculatory Encephalopathy of Mixed Genesis

Authors

  • Vladyslav G. Babiychuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Viktoria S. Aidarova V.N. Karazin Kharkiv National University, Kharkiv
  • Ivan I. Lomakin Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Olga V. Kudokotseva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Nataliia V. Roy Kharkiv National Medical University, Kharkiv
  • Olga V. Naumova Kharkiv National Medical University, Kharkiv
  • Оlena S. Protsenko V.N. Karazin Kharkiv National University, Kharkiv

DOI:

https://doi.org/10.15407/cryo31.02.151

Keywords:

spontaneously hypertensive rats, dyscirculatory encephalopathy, chronic alcohol intoxication, rhythmic craniocerebral hypothermia, cryopreserved nucleated cord blood cells, brain

Abstract

The study examines the morphological changes that occur in brain of SHR rats on the background of developing hypertensive dyscirculatory encephalopathy (DE), complicated by chronic alcohol intoxication (CAI). CAI has been proven to potentiate the progressive aging of brain in rats with hypertensive DE by aggravating its course and to lead to the development of DE of mixed genesis. The effectiveness of a single and combined use of rhythmic craniocerebral hypothermia (rCCH) and the introduction of cryopreserved cord blood nucleated cells (cCBNCs) to reduce the progressive vascular lesions of brain and the effects of chronic ischemia in animals with DE of mixed genesis. It has been proven that the combined use of rCCH and cCBNCs in the rats with a mixed DE had a more pronounced positive effect on morphological structure of brain tissues and vessels than their single use. There was a further decrease in the signs of dystrophic, destructive and pathologically altered compensatory adaptive processes in brain on the background of a significant decrease in neuroglial index, stimulation of angiogenesis, complete or partial absence of signs of smooth muscle cell hyperplasia, acute hemorrhages and ischemic strokes, spasms of arterial vessels’ walls and plethory in veins, that contributed to restoration of brain microhemocirculation and cytoarchitectonics.

Probl Cryobiol Cryomed 2021; 31(2): 151–160

 

Author Biographies

Vladyslav G. Babiychuk , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

Ivan I. Lomakin , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

Olga V. Kudokotseva , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

References

Achyut BR, Varma NRS, Arbab AS. Application of umbilical cord blood derived stem cells in diseases of the nervous system. J Stem Cell Res Ther. [Internet]. 2014 May 7 [cited 20.02.2018]; 4(5): 1000202. Available from: https://www.longdom.org/open-access/application-umbilical-cord-blood-derivedstem-cells-diseases-nervous-system-2157-7633.1000202.pdf. CrossRef

Aidarova VS, Babiichuk VG, Kudokotseva OV, et al. Experimental substantiation of therapeutic hypothermia and cell therapy application at dyscirculatory encephalopathy in SHR rats. Part 1. Spontaneously hypertensive SHR rats as a model of dyscirculatory encephalopathy. Probl Cryobiol Cryomed 2018; 28(3): 224-36. CrossRef

Aidarova VS, Babiichuk VG, Kudokotseva OV, et al. Experimental substantiation of therapeutic hypothermia and cell therapy application at dyscirculatory encephalopathy in SHR rats. Part 2. Structural changes in brain tissue. Probl Cryobiol Cryomed 2019; 29(1): 58-72. CrossRef

Aidarova VS, Naumova OV, Kudokotseva OV, et al. [Brain structure of SHR rats with genetically determined arterial hypertension]. World of Medicine and Biology. 2018; (2): 115-9. Russian. CrossRef

Babiychuk GA, Marchenko VS, Lomakin II, Belostotskiy AV. [Neurophysiological processes of cooled brain]. Kyiv: Naukova dumka; 1992. Russian.

Coca A. Hypertension and brain damage. New York: Springer; 2016. 329 p. CrossRef

De Deyn PP, Dam DV, editers. Animal models of dementia. New York: Humana Press; 2011. 732 р. CrossRef

Grishchenko VI, Kovalov GA, Petrenko AYu, et al. [Regenerative-plastic therapy of alcoholic visceropathies]. Kyiv: Naukova Dumka, 2010. Russian.

Gusev E, Konovalov A, Skvortsova V. [Neurology. National guidance]. Moscow: All-Russian Society of Neurologists; 2015. Russian.

Hachinski V. World stroke day proclamation. Stroke. 2008; 39(10): 2409-20. CrossRef

Jaing TH. Umbilical cord blood: a trustworthy source of multipotent stem cells for regenerative medicine. Cell Transplant. 2014; 23(4-5): 493-6. CrossRef

Lomakin II. Substantiation of medical cooling methods in therapy of chronic alcoholism. Problems of Cryobiology 2008; 18(3): 383-5. Full Text

Mishchenko TS. [Epidemiology of cerebrovascular diseases and organization of care for stroke patients in Ukraine]. Ukrainian newsletter of neurology. 2017; 25(90): 22-4. Russian.

Downloads

Published

2021-07-15

How to Cite

Babiychuk, V., Aidarova, V., Lomakin, I., Kudokotseva, O., Roy, N., Naumova, O., & Protsenko О. . (2021). Influence of Hypothermia and Cell Therapy on Structural Changes in Brain Tissues of Rats with Dyscirculatory Encephalopathy of Mixed Genesis. Problems of Cryobiology and Cryomedicine, 31(2), 151–160. https://doi.org/10.15407/cryo31.02.151

Issue

Vol. 31 No. 2 (2021): Probl Cryobiol Cryomed

Section

Theoretical and Experimental Cryobiology

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).