Blood Characteristics in Dogs During Treatment of Babesiosis Using Transfusion of Cryopreserved Erythrocytes
DOI:
https://doi.org/10.15407/cryo31.04.304Keywords:
babesiosis, canine erythrocytes, transfusion, cryopreservation, cryoprotectantAbstract
Babesiosis is one of the most common protozoan diseases in dogs, which in the absence of the necessary veterinary care often leads to the animal death. The changes of hematological parameters in dogs during complex treatment of babesiosis and transfusion of cryopreserved erythrocytes were evaluated in the research. Erythrocytes were frozen using 20% DMSO by immersion into liquid nitrogen. Transfusion of cryopreserved erythrocytes together with antiparasitic chemotherapy has been shown to promote rapid recovery of hematological parameters of animal blood (hemoglobin, hematocrit and erythrocyte counts), as well as improves their well-being, promotes faster recovery. Cryopreserved with DMSO erythrocytes can be recommended for long-term storage of blood at cryobanks.
Probl Cryobiol Cryomed 2021; 31(4): 304–315
References
Aktaran Bala D, Özcan M. The effects of freezing on long-term storage of canine erythrocytes. Pol J Vet Sci. 2016; 19(2):401-6. CrossRef
Contreras TJ, Lindberg JR, Lowrie GB, et al. Liquid and freeze-preservation of dog red blood cells. Transfusion. 1979; 19 (3): 279-92. CrossRef
Соtter S.M. Advances in veterinary science and comparative medicine: comparative transfusion medicine, V. 36. San Diego: Academic Press; 1991. 343 p.
DeLuca LA, Glass SG, Johnson RE, Burger M. Description and evaluation of a canine volunteer blood donor program. J Appl Anim Welf Sci. 2006; 9 (2): 129-41. CrossRef
Denisova ON, Zhegunov GF, Babijchuk LA. Cryopreservation of animal's erythrocytes under dimethyl sulfoxide, polyethylene oxide and glycerol protection. Problems of Cryobiology. 2005; 15 (2): 195-201. Full Text
Denysova ON, Zhegunov GF, Zemlianskykh NG. [Resistance of erythrocytes of animals against the influence of low temperatures.] Naukovyi Vіsnyk Natsіonalnogo Agrarnogo Unіversytetu. 2008; 126: 67-75. Ukraine
Dobry E, Novac J. Hematologie a transfuzni sluzba, Zdravotnicke actuality 211, Praha: Avicenum; 1987. 188 p.
Graham JE, Meola DM, Kini NR, Hoffman AM. Comparison of the effects of glycerol, dimethyl sulfoxide, and hydroxyethyl starch solutions for cryopreservation of avian red blood cells. Am J Vet Res. 2015; 76 (6): 487-93. CrossRef
Kim H, Itamoto K, Une S, et al. Application of phosphoenolpyruvate into canine red blood cell cryopreservation with hydroxyethyl starch. CryoLetters. 2005; 26 (1): 1-6. PubMed
Kim H, Tanaka S, Une S, et al. A comparative study of the effects of glycerol and hydroxyethyl starch in canine red blood cell cryopreservation. J Vet Med Sci. 2004; 66 (12): 1543-7. CrossRef
Kostyaev AA, Martusevich AK, Andreev AA. [Toxicity of cryoprotectants and cryoconservants on their basis for blood components and bone marrow]. Nauchnoe obozrenie. Meditsinskie nauki.2016; (6): 54-74. Russian.
Langer R, Albrecht R, Hempel K, et al. Characterization of 24-hour survival rate and duration of survival of hydroxyethyl starch cryopreserved erythrocytes after autologous transfusion in the dog. Infusionsther Transfusionsmed. 1994; 21 (6): 393-400. CrossRef
Leisewitz AL, Guthrie AJ, Berry WL. Evaluation of the effect of whole - blood transfusion on the oxygen status and acid-base balance of Babesia canis infected dogs using the oxygen status algorithm. J S Afr Vet Assoc. 1996; 67 (6): 20-6.
Meyer D, Kharvy D. [Veterinary laboratory medicine. Interpretation and diagnosis] Moskow: Sofyon; 2007. 456. Russian.
Pogozhykh D, Pakhomova Y, Pervushina O, et al. Exploring the possibility of cryopreservation of feline and canine erythrocytes by rapid freezing with penetrating and non-penetrating cryoprotectants. PLoS One One [Internet]. 2017 Jan 10 [cited 2020 May 29]; Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169689 CrossRef
Rozanski E, de Laforcade AM. Transfusion medicine in veterinary emergency and critical care medicine. Clin Tech Small Anim Pract. 2004; 19: 83-7. CrossRef
Sant'Anna Leal PD, Flausino W, Gomes Lopes CW. [Diagnosis of concomitant infections due to Neospora caninum, Babesia canis and Erhlichia spp. in adult canine Golden Retriever breed - Case report]. Rev Brasileira de Med Vet. 2012; 34 (1): 47-51. Portuguese.
.
Singbartl K, Langer R, Henrich A. Altered membrane skeleton of hydroxyethylstarch-cryopreserved human erythrocytes. Cryobiology. 1998; 36 (2): 115-23. CrossRef
Smirnov AA, Fedosov AA, Klimov PV. [Drug Pyro-stop - present and effective solution in the fight against animal piroplasmidosis.] Voprosy' normativno-pravovogo regulirovaniya v veterinarii. 2011; 1: 45-7. Russian.
Sputtek A, Langer R, Schmid H, et al. Cryopreservation of erythrocytes with hydroxyethyl starch. In vitro results leading to an autologous retransfusion model in the dog. Beitr Infusionsther. 1992; 30: 292-6.
Swanson BN. Medical use of dimethyl sulfoxide (DMSO).Rev Clin Basic Pharm. 1985; 5(1-2):1-33.
Wardrop KJ, Owen TJ, Meyers KM. Evaluation of an additive solution for preservation of canine red blood cells. J Vet Intern Med. 1994; 8: 253-7. CrossRef
Weiss DJ, Wardrop KJ, editors. Schalm's veterinary hematology. Singapore: Wiley; 2010. 1232 p.
Wintrobe MM. Principles of hematologic examination. In: Wintrobe MM, editor. Clinical hematology. 8th ed. Philadelphia: Lea & Febiger; 1981. p.7-19.
Wong LK, Reinertson EL. Clinical considerations of dimethyl sulfoxide. Iowa State University Veterinarian. 1984; 46 (2): 89-95.
Zemlianskykh NG, Babijchuk LA. [Spatial-conformational modifications of proteins in membrane]. Biologіscheskie membrany.2019;36 (2): 125-36. Russian. CrossRef
Zemlianskykh NG, Denisova ON. [Changes in erythrocyte membrane-cytoskeleton complex, induced by dimethylsulfoxide, polyethyleneglycol, and low temperature]. Biofizika. 2009; 54 (4): 490-6. Russian. CrossRef
Zhegunov GF, Denisova ON. [Permeability of mammalian erythrocytes for the molecules of glycerol and DMSO and the level of cellular viability after freezing-thawing.] Dopov Nac akad nauk Ukr. 2010; 12: 139-43. Russian.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
