Evaluation of Mitotic Activity of Bone Marrow Under Short-Term Hypothermia
Keywords:hypothermia, mitotic index, bone marrow, medicinal leech application, actively proliferating cells
In this research, we have studied the mitotic activity of bone marrow when applying medicinal leeches and short-term hypothermia. The bone marrow mitotic indices were compared within an immediate examination of the thigh removed from the body and during a short-term hypothermia. As a result, no statistical differences were found between the mitotic levels during immediate examination and a short-term hypothermia in the intact animals and those after medicinal leech application, that evidenced to a hypothermal fixation of initial level of this index, preservation of biomembrane physiological state prior to hypotonic stress and cell morphology. The used here short-term hypothermia on bone marrow cells may be included in the multi-profile algorithm of laboratory analysis when studying a mitotic index in actively proliferating cells.
Probl Cryobiol Cryomed 2022; 32(2):081–091
Aminov R, Aminova A, Makyeyeva L. Morphological parameters of spleen and thymus of the male rats on the basis of the hirudological influence of Hirudo verbana. Ann Parasitol. 2022; 68(1): 55-60. PubMed
Aminov R, Frolov A, Aminova A. The effect of the biologically complex of a medical leech active substances on the immunosuppressive state of rats. Jordan J Biol Sci. 2022; 15(2): 257-61. CrossRef
Aminov RF, Frolov AK. Influence of ectoparasite - Hirudo verbana on morphogenetic reactions of the host organism - rattus. Curr Trends Immunol. 2017; 18: 107-17.
Aminov RF, Frolov AK. The impact of fetal load of Hirudo verbana saline extract antigens on morphometrical, hematological and immunological parameters of rats in the early stages of post-embryonic development. Ann Parasitol. 2018; 64(1): 13-20. PubMed
Aminov RF, Frolov OK. [Proliferative activity of bone marrow cells of rats under the influence of biologically active substances of a medicinal leech]. Regulatory Mechanisms in Biosystems. 2017; 8(4): 501-5. Ukrainian. CrossRef
Aminov RF, Syrtsov VK, Fedosieieva OV, Frolov AK. [Morphological parameters of spleen and thymus of the rats after the influence of saline extracts Hirudo verbana.] World of Medicine and Biology 2019; (4): 183-7. Ukrainian. CrossRef
Berkalo LV, Bobovich OV, Bobrova NO. [Methods of clinical and experimental research in medicine]. Poltava: Polimet; 2003. 320 p. Ukrainian.
Frenkel MA, Chigrinova EV, Kupryshina NA, Pavlovskaia AI. [Diagnostic value of study of bone marrow trepanobiopsy imprints in patients with non-Hodgkin's lymphomas.] Klin Lab Diagn. 2007; (11):44-7. Russian. PubMed
He N, Zhang L, Cui J, Li Z. Bone marrow vascular niche: home for hematopoietic stem cells. Bone Marrow Res. [Internet]. 2014 Apr 14 [cited 14.02.2021]; 2014: 128436. Available from: https://www.hindawi.com/journals/bmr/2014/128436/ CrossRef
Ivanov OS, Bagmut IYu, Tsapko GV, Sklyar SI. [Influence of toxic and subtoxic doses of diclofenac sodium on mechanisms of differentiation of granulocytary row cells, undifferentiated blasts and mitotic cells in the bone marrow of rats]. Ukrainskyi Zhurnal Medytsyny, Biolohii ta Sportu. 2019; 22(6): 46-51. Ukrainian. CrossRef
Lyndina YuM, Sikora VV, Hyriavenko NI, et al. [The study of the protective properties of vitamin E on bone marrow of rats in condition of the increased intake of heavy metals salts]. Reports of Vinnytsia National Medical University. 2019; 23(1): 59-64. Ukrainian. CrossRef
Ohiienko SL, Bondar AYu, Ivanov EG, Bozhkov AI. Liver fibrosis has a different effect on the "lifespan" of lymphocytes and neutrophils in the in vitro system isolated from the bone marrow of young and old rats. MOJ Gerontol Ger. 2019; 4(1): 36-40.
Ojo PО, Babayi H, Olayemi IK, et al. Anti-tubercular activities and molecular characterization of salivary extract of leech (Hirudo medicinalis) against mycobacterium tuberculosis. J Tuber Res. 2018; 6(1): 1-9. CrossRef
Praise OO, Babayi H, Olayemi IK, et al. Effects of leech salivary extract (lse) on indices of liver function in rats. Clin Diagn Pathol. 2018 Jan 31 [cited Mar 10 2021]; 2(1): 1000124. Available from: https://www.oatext.com/Effects-of-leech-salivary-extract-lse-on-indices-of-liver-function-in-rats.php#Article CrossRef
Shakouri A, Adljouy N, Abdolalizadeh J. Anti-cancer activity of liposomal medical leech saliva extract (LSE). In: Proceedings of the 3rd World Congress on Recent Advances in Nanotechnology (RAN'18). Budapest, Hungary, April 10-12, 2018. Budapest; 2018. Paper No. NDDTE 102. [Internet] [cited 12.02.2021]. Available from: https://avestia.com/RAN2018_Proceedings/files/paper/NDDTE/NDDTE_102.pdf
Sikora VZ, Prihodko OO. [Structural and functional characteristics of blood of senile rats under the action of heavy metal salts]. Ukrainskyi Morfolohichnyi Almanakh. 2010; 8(2): 191-3. Ukrainian.
Sobczak N, Kantyka M. Hirudotherapy in veterinary medicine. Ann Parasitol. 2014; 60(2): 89-92. PubMed
Tavian M, Péault B. Embryonic development of the human hematopoietic system. Int J Dev Biol. 2005; 49(2-3): 243-50. CrossRef
Travlos GS. Histopathology of bone marrow. Toxicol Pathol. 2006; 34(5): 566-98. CrossRef
Yu G, Baeder DY, Regoes RR, Rolff J. Combination effects of antimicrobial peptides. Antimicrob agents chemother. 2016; 60(3): 1717-24. CrossRef
How to Cite
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).