Osmotic Characteristics of Erythrocytes After Freezing with Composition-Modified Cryopreservatives
DOI:
https://doi.org/10.15407/cryo32.02.104Keywords:
erythrocytes, freezing, glycerol, 1,2-propanediol, osmotic stressAbstract
Here, we have studied the osmotic parameters of erythrocytes frozen in the composition-modified media with glycerol and 1,2-propanediol. A decreased glycerol concentration in a cryopreservative and an increased content of impermeable protective component sorbitol were established to affect the erythrocyte characteristics at different stages of freezing and hypothermic storage. Reduction of 1,2-propanediol and NaCl concentrations when augmenting the sucrose content in cryopreservative agent enabled changing a two-stage mode of erythrocyte freeze-thawing to the single-stage one. The presented here modification of cryoprotective medium allowed to diminish the level of damage to erythrocytes during freeze-thawing-washing out, that might prevent the inflammation development in a body when transfusing these cells.
Probl Cryobiol Cryomed 2022; 32(2):092–103
References
Bakhach J. The cryopreservation of composite tissues: Principles and recent advancement on cryopreservation of different type of tissues. Organogenesis. 2009; 5(3): 119-26. CrossRef
Bogner V, Keil L, Kanz KG, et al. Very early posttraumatic serum alterations are significantly associated to initial massive RBC substitution, injury severity, multiple organ failure and adverse clinical outcome in multiple injured patients. Eur J Med Res. 2009; 14(7): 284-91. CrossRef
De Loecker R, Goossens W, Van Duppen V. Osmotic effects of dilution on erythrocytes after freezing and thawing in glycerol-containing buffer. Cryobiology. 1993; 30(3): 279-85. CrossRef
Guchok VM, Vorotilin AM, Lougovov VI, Shrago MI. Therapeutic efficiency of RBC, cryopreserved under protection of a "Propanediosaccharole" preparation. Problems of Cryobiology. 1994; (4): 43-6.
Henkelman S, Noorman F, Badloe JF, Lagerberg JW. Utilization and quality of cryopreserved red blood cells in transfusion medicine. Vox Sang. 2015; 108: 103-12. CrossRef
Hod EA, Zhang N, Sokol SA, et al. Transfusion of red blood cells after prolonged storage produces harmful effects that are mediated by iron and inflammation. Blood. 2010; 115(21): 4284-92. CrossRef
Lelkens CC, Noorman F, Koning JG, et al. Stability after thawing of RBCs frozen with the high- and low-glycerol method. Transfusion. 2003; 43(2): 157-64. CrossRef
Miller RH, Mazur P. Survival of frozen-thawed human red cells as a function of cooling and warming velocities. Cryobiology. 1976; 13(4): 404-14. CrossRef
Pakhomova YuS, Kompaniets AM, Kuleshova LG. Transformation of erythrocytes during cryopreservation with oxyethylated glycerol derivatives with n=25 and n=30 polymerization degree. Probl Сryobiol Cryomed. 2016; 26(4): 349-60. CrossRef
Ramazanov VV. Effect of combined media on damage of erythrocytes, frozen with different hematocrit values. Problems of Cryobiology. 2006; 16(2): 155-63. Full Text
Ramazanov VV, Volovelska YeL, inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [The method of cryopreservation of human erythrocytes]. Patent of Ukraine №114838A. March 27 2017. Ukrainian.
Rall WF, Mazur P, Souzu H. Physical-chemical basis of the protection of slowly frozen human erythrocytes by glycerol. Biophys J. 1978; 23(1):101-20. CrossRef
Spitalnik SL. Stored red blood cell transfusions: iron, inflammation, immunity, and infection. Transfusion. 2014; 54(10): 2365-71. CrossRef
Thuret I. Post-transfusional iron overload in the haemoglobinopathies. C R Biol. 2013; 336(3): 164-72. CrossRef
Valeri CR, Ragno G. An approach to prevent the severe adverse events associated with transfusion of FDA-approved blood products. Transfus Apher Sci. 2010; 42(3): 223-33. CrossRef
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
