Effect of Cryopreservation on Viability, Immunophenotype and Differentiation Properties of Mesenchymal Stromal Cells of Early Organogenetic Stage
Keywords:cryopreservation, mesenchymal stromal cells, viability, immunophenotype, differentiation
The effect of cryopreservation by slow program cooling on the viability, immunophenotypic and differentiative properties of mesenchymal stromal cells (MSCs) of early organogenesis stage was studied. It was shown that after cryopreservation under the protection of 10% dimethyl sulfoxide (Me2SO) at the cooling rate of 1Â°C/min down to â€“80Â°C with the following plunging into liquid nitrogen the cell viability assessed by trypan blue staining was 88.4 Â± 1.7%. The metabolic activity of cells assessed by Alamar Blue reduction assay decreased after cryopreservation by 15%. Immunophenotypic analysis of MSCs after 4â€“12 passages showed that 95% of cells expressed the markers CD29, CD44, CD73, CD105 prior to and after cryopreservation. The number of CD34â€“, CD38â€“ and CD45â€“ cells was more than 95%. After cryopreservation MSCs maintained their capacity for induced differentiation into osteogenic and adipogenic directions. Slow cooling with Me2SO as cryoprotectant was observed to stimulate differentiation of MSCs.
How to Cite
Copyright (c) 2020 N. A. Trufanova, Yu. A. Petrenko, A. Yu. Petrenko
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).